Abstract:ObjectiveTo explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress.MethodsRNA was extracted from Raw264.7 cell

3T3 cell and 10T1/2 cell after 5 hours stimulated with 5 μg/mL LPS. At the same time

to set up normal control group (untreated by LPS)

and RNA of IFIT1 was detected by RT-PCR.Totally 20 C57/BL6 mice were randomly divided into 4 groups

namely 0 h group

1 h group

4 h group and 12 h group. The mice were given 12 Gray60Co full-body exposure once

then liver IFIT1 was detected by western blot.ResultsStimulated with LPS for 5 hours

IFIT1 was induced expression in Raw264.7 cell

3T3 cell and 10T1/2 cell. The expression of normal control group was negative.The level of IFIT1/Actin increased significantly 1 hour after radiation injury

and it reached the peak 12 hours after radiation injury (P＜0.01).ConclusionLPS can stimulated a variety of cell lines expressed IFIT1

prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia.IFIT1 of liver tissue increased significantly during the early stage in radiation mice.