幼兔重度肺冲击伤动物模型的建立

梁  毅; 代  维; 麻  超; 张潇俊; 游  昕; 周继红

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幼兔重度肺冲击伤动物模型的建立

基础研究 | 更新时间：2023-05-22

- 幼兔重度肺冲击伤动物模型的建立
- Study on severe blast lung injury model of baby rabbits
- 局解手术学杂志 2016年第5期
- 作者机构：
  
  1. 第三军医大学大坪医院野战外科研究所四室,重庆,400042
  2. 成都军区总医院急诊科，四川成都 6100384543.南京军区医院心胸外科,江苏,南京,210000
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81471865）;南京军区重点科研课题（ZX14 2014）
- DOI：
  中图分类号：
- 纸质出版日期：2016，
扫描看全文
梁毅, 代维, 麻超, 等. 幼兔重度肺冲击伤动物模型的建立[J]. 局解手术学杂志, 2016,(5).

Liang Yi, Dai Wei, Ma Chao, et al. Study on severe blast lung injury model of baby rabbits[J]. null, 2016,(5).
梁毅, 代维, 麻超, 等. 幼兔重度肺冲击伤动物模型的建立[J]. 局解手术学杂志, 2016,(5). DOI：

Liang Yi, Dai Wei, Ma Chao, et al. Study on severe blast lung injury model of baby rabbits[J]. null, 2016,(5). DOI：

摘要

[摘要]目的建立稳定的幼兔重度肺冲击伤实验动物模型，为未成熟肺冲击伤机制和肺损伤救治研究提供理想的动物模型。方法致伤驱动压初筛：选取4周龄新西兰白兔16只，随机分为驱动压4.0 MPa组和4.5 MPa组，采用BST-Ⅰ型生物激波管，以相应的驱动压力致伤，对比2组肺冲击伤伤情。肺冲击伤伤情特点观察：选取4周龄新西兰白兔48只，随机分为正常对照组（8只）和冲击伤组（40只），依前面研究结果，冲击伤组采用4.5 MPa驱动压进行致伤；分别于伤即刻（0 h）、2 h、4 h、6 h、12 h、24 h、48 h及72 h观测动物生命体征和生理指标、肺大体解剖和光镜病理、测定肺组织含水量等。结果致伤驱动压初筛：2组动物均存活，4.0 MPa组冲击波超压为（328.16±4.78）kPa，重度肺冲击伤率为12.5%，AIS评分（3.38±0.52）分；4.5 MPa组冲击波超压为（395.04±11.74）kPa，重度肺冲击伤率为87.5 %，AIS评分（4.13±0.64）分。肺冲击伤伤情特点观察：致伤动物均存活，伤后即刻出现持续约0.5 h的精神萎靡等情况，随后动物呼吸和心率等加快，肺出现广泛片状出血和水肿，肺含水量显著增加，多为重度冲击伤，AIS评分（3.98±0.55）分，肺光镜病理以肺组织断裂、出血、水肿，伴炎细胞浸润为主。结论利用BST-Ⅰ型生物激波管，采用4.5 MPa驱动压可建立稳定的4周龄新西兰白兔重度冲击伤模型，此模型伤情稳定，可用于未成熟肺冲击伤机制和肺损伤救治的实验研究模型。

Abstract

Abstract: ObjectiveTo establish an animal model of severe blast lung injury in baby rabbits

and to provide a way to study the characteristic and treatment of blast lung injury in minors.MethodsRandomly selected sixteen 4-weeks old New Zealand white rabbits

and the blast lung injuries were made by BST-Ⅰ biological shock tube with different drive pressure (4.0Mpa and 4.5MPa) respectively. Thencompared the injury severity of the 4.0Mpa group and the 4.5MPa group.Selected forty-eight 4-weeks old New Zealand white rabbits and divided them into the control group (8 rabbits) and the blast lung injury group (40 rabbits)Rabbits in the blast lung injury group were injured with 4.5MPa drive pressure.Observed the vital signs

physiological index

gross anatomy of the lung

pathology

and pulmonary water content at the time of injury immediately (0 hour)

2 hours

4 hours

6 hours

12 hours

24 hours

48 hours and 72 hours after the injury.ResultsRabbits in the 4.0Mpa group and the 4.5MPa group were all alive.The overpressure of blast wave of the 4.0Mpa group was (328.16±4.78)kPa

rate of severe pulmonary defense was 12.5%

and the AIS score was (3.38±0.52)points.In the 4.5MPa group

the overpressure of blast wave was (395.04±11.74)kPa

rate of severe pulmonary defense was 87.5%

and the AIS score was (4.13±0.64)points.Rabbits in the control group and the blast lung injury group were all alive.The spirits of rabbits were drooping immediately after injury

and it last about 0.5 hour.Then the breathing and heart rate was accelerated

pulmonary water content was increased significantly

and there were extensive hemorrhage and edema in the lung.Most of the rabbits suffered severe lung injury

and the AIS score was (3.98±0.55) points.Lung tissue rupture

hemorrhage

edema

and inflammatory cells infiltration were the main pathological manifestations under light microscopy.ConclusionThe model of severe blast lung injury in baby rabbits could be established with BST-Ⅰbiological shock tube and drive pressure of 4.5 MPa.It is relatively simple

easily controllable and highly repeatable，which can be used as a feasible model for the study of blast lung injury.

关键词

肺冲击伤幼兔动物模型生物激波管

Keywords

blast lung injurybaby rabbitanimal modelbiological shock tube

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