Objective To identify the route of the female anterior pelvic floor reconstruction which is applied to treat anterior vaginal wall prolapse（AVWP），and to decrease the complication of procedure. Methods Three fresh cadavers were injected colored latex into radial artery.After 5 days

the anterior pelvic floor reconstruction were performed respectively when lactoprene had solidified.The distances between the trocars and related vascular

nerves and hymen were measured. Results There was no complication during all procedures.The distances between No.1 needle and the posterior branch of the obturator nerve

anterior branch of the obturator artery

posterior branch of the obturator artery

obturator canal

internal pudendal vessels and nerves

and hymen were (2.6±0.2)cm

(2.1±0.3)cm

(1.4±0.1)cm

(2.1±0.2)cm

(1.2±0.2)cm and (2.0±0.2)cm respectively.The distances between No.2 needle and the posterior branch of the obturator nerve

anterior branch of the obturator artery

posterior branch of the obturator artery

obturator canal

internal pudendal vessels and nerves

and hymen were (3.0±0.3)cm

(2.9±0.2)cm

(0.6±0.2)cm

(2.5±0.3)cm

(0.8±0.2)cm and (6.1±0.4)cm respectively. Conclusion The AVWP can be treated with female anterior pelvic floor reconstruction by enhancing the supporting functions of level Ⅰ and Ⅱ.In order to reduce the injury of important vascular and nerve

the patients should be placed in lithotomy position and fully abduct thighs

and the appropriate position to insert and perform outsidein procedure should be selected.