血浆hsa-miR-19a-3p与急性冠脉综合征相关性研究及其生物信息学分析

黄浩; 陈文江; 陈国柱; 秦芳; 刘长召; 王玲; 华晓芳

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血浆hsa-miR-19a-3p与急性冠脉综合征相关性研究及其生物信息学分析

基础研究 | 更新时间：2023-05-22

- 血浆hsa-miR-19a-3p与急性冠脉综合征相关性研究及其生物信息学分析
- Correlation between plasma hsa-miR-19a-3p and acute coronary syndrome and its bioinformatics analysis
- 局解手术学杂志 2019年第4期页码：267
- 作者机构：
  
  1. 恩施土家族苗族自治州中心医院心血管病中心,湖北,恩施,445000
  2. 重庆医科大学附属第二医院心血管内科,重庆,400010
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81270262）
- DOI：
  中图分类号：
- 纸质出版日期：2019，
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黄浩, 陈文江, 陈国柱, 等. 血浆hsa-miR-19a-3p与急性冠脉综合征相关性研究及其生物信息学分析[J]. 局解手术学杂志, 2019,(4):267.

HUANG Hao, CHEN Wen-jiang, CHEN Guo-zhu, et al. Correlation between plasma hsa-miR-19a-3p and acute coronary syndrome and its bioinformatics analysis[J]. null, 2019,(4):267.
黄浩, 陈文江, 陈国柱, 等. 血浆hsa-miR-19a-3p与急性冠脉综合征相关性研究及其生物信息学分析[J]. 局解手术学杂志, 2019,(4):267. DOI：

HUANG Hao, CHEN Wen-jiang, CHEN Guo-zhu, et al. Correlation between plasma hsa-miR-19a-3p and acute coronary syndrome and its bioinformatics analysis[J]. null, 2019,(4):267. DOI：

摘要

目的分析hsa-miR-19a-3p与急性冠状动脉综合征(ACS)的相关性，并探讨其可能机制。方法选择2014年3月至2015年9月湖北省恩施土家族苗族自治州中心医院心血管病中心收治的118例冠心病患者作为研究对象，并按照冠心病分类分为STEMI组（34例）、NSTEMI组（25例）、UAP组（22例）和SCAD组（37例），采用实时荧光定量PCR检测血浆hsa-miR-19a-3p的表达，采用生物信息学分析其可能靶基因、生物信息学功能和可能结合非编码RNA（如miRNA、lncRNA、circRNA等）；采用酶联免疫吸附法（ELISA）检测低密度脂蛋白受体相关蛋白2（LRP2）的表达。结果 STEMI组和NSTEMI组血浆hsa-miR-19a-3p水平较UAP组和SCAD组明显升高，差异具有统计学意义（P<0.05）；hsa-miR-19a-3p的靶基因可能为LRP2，其生物信息功能主要与脂蛋白生物合成过程、脂肪因子信号通路、白介素信号通路、血小板源生长因子信号通路、缺氧诱导因子激活缺氧反应、血管生成、丝/苏氨酸激酶信号通路、丝裂素活化蛋白激酶信号通路、转化生长因子β信号通路和趋化因子信号通路等有关；hsa-miR-557、hsa-miR-507、RP1170C1.1、CTD-2147F2.2、C9orf106、C1orf198_hsacircRNA8985、FGFR3_hsacircRNA5885、CAMK2N1_hsa-circRNA8340、TGFBR3_hsa-circRNA859和BCL3_hsacircRNA4124等可能为其“海绵”；血浆LRP2水平，STEMI组（1 511±212.9）ng/mL、NSTEMI组（1 436±400.0)ng/mL和UAP组（587.5±176.0)ng/mL较SCAD组（367.4±47.69）ng/mL明显升高，差异具有统计学意义（P<0.05）；血浆hsa-miR-19a3p与LRP2 Pearson相关系数为0.488 1（95%CI：0.312 5~0.631 4，P=0.000 4）。结论 hsa-miR-19a-3p与ACS密切相关，通过调控hsa-miR-19a-3p的靶基因LRP2而导致ACS的发生，可能与脂质代谢密切相关。

Abstract

Objective To investigate the relationship of hsa-miR-19a-3p and acute coronary syndrome(ACS)

and to explore its possible mechanism. Methods A total of 118 patients who were admitted into hospital central hospital of Enshi autonomous prefecture were selected as the objects

and they were divided into the STEMI group (n=34)

the NSTEMI group (n=25)

the UAP group (n=22) and SCAD group (n=37) according to the classification of coronary heart disease.The expression of hsa-miR-19a-3p was detected by the realtime fluorescence quantitative PCR. The target genes

bioinformatics function and the noncoding RNAs (miRNA

lncRNA

circRNA

etc) of hsa-miR-19a-3p′s were analyzed by the bioinformatics techniques.The expression of low density lipoprotein receptorassociated protein 2 (LRP2) was detected by enzymelinked immunosorbent assay (ELISA). Results Plasma hsa-miR-19a-3p in STEMI group and NSTEMI group were significantly higher than the UAP group and SCAD group(P<0.05). LRP2 may be a target gene of hsa-miR-19a-3p.And its main function of biological information may be connected with the biosynthesis of lipoprotein

fat factor signaling pathways

interleukin signaling pathways

plateletderived growth factor signaling pathway

oxygen reaction activated by hypoxia inducing factor

angiogenesis

serine/threonine kinase signaling pathways

mitogenactivated protein kinase signaling pathways

transforming growth factor β signaling pathways and chemokine signaling pathway and so on.Hsa-miR-557

hsa-miR-507

RP11-70C1.1

CTD-2147F2.2

C9orf106

C1orf198_hsacircRNA8985

FGFR3_hsacircRNA5885

CAMK2N1_hsacircRNA8340

TGFBR3_hsacircRNA859 and BCL3_hsacircRNA4124 may severed as its "sponge". The plasma LRP2 levels in STEMI group (1 511±212.9) ng/mL

NSTEMI group (1 436±400.0) ng/mL

UAP group (587.5±176.0) ng/mL were significantly higher than SCAD group (367.4±47.69) ng/mL

and the differences were statistically significant (P<0.05). The Pearson correlation coefficient between plasma hsa-miR-19a-3p and LRP2 was 0.4881 (95%CI:0.312 5~0.631 4

P=0.000 4). Conclusion HsamiR19a3p may be closely associated with ACS by regulating LRP2 through lipid metabolism process.

关键词

hsa-miR-19a-3p急性冠状动脉综合征低密度脂蛋白受体相关蛋白2脂质代谢生物信息学

Keywords

hsa-miR-19a-3pacute coronary syndromelow density lipoprotein receptor-associated protein 2lipid metabolismbioinformatics

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