Objective To study the characteristics of biological behavior changes of Tca8113 and HSC2 cell lines in head and neck squamous cell carcinoma after exposed to isoflurane in vitro

so as to provide theoretical guidance for the surgery. Methods Tca8113 and HSC2 cell lines were cultured in vitro for 72 hours after exposed to 2.0% isoflurane 3 hours and 6 hours respectively. MTT test was used to detect the effect of 2% isoflurane on cell proliferation. Transwell test was used to detect the effect of 2% isoflurane on cell invasion ability. Results MTT and Transwell indicated that the proliferation and invasion ability of Tca8113 and HSC2 cell lines showed significant increase after exposed to 2.0% isoflurane 3 to 6 hours

and the differences were statistically significant compared with the control group without isoflurane exposure (P<0.05). Conclusion Isoflurane can increase the malignancy of head and neck squamous cell carcinoma cell lines in vitro. It is suggested that isoflurane might enhance tumor development and promote metastasis of tumor cells in patients with head and neck squamous cell carcinoma.