Objective To clarify the mechanism of BMP2/4 involvement in intestinal mucosal barrier injury under ischemia reperfusion. Methods The cell injury model was established by hypoxia and animal model of rat with intestine ischemiareperfusion injury

then the westernblot and immunofluorescence were used to detect the change of BMP signal pathway.Westernblot and immunofluorescence were used to detect the effect of BMP2 and BMP4 on the activation of NF-κB signaling pathway. RT-PCR was used to detect the expression of TNF-a mRNA and IL-6 mRNA in IEC-6 cells.Westernblot was used to detect the effect of IEC6s with exogenous BMP2 or BMP on change of occludin. Results The expression levels of BMP2 and BMP4 were upregulated in intestinal epithelial cells after hypoxia and ischemiareperfusion injury.The NF-κB signaling pathway in intestinal epithelial cells was activated by BMP2 and BMP4

which resulted in the release of TNF-a and IL-6

and increased permeability of intestinal mucous membrane barrier. Meanwhile

BMP2 and BMP4 could decrese the expression of occludin through the activation of NF-κB signaling pathway

damaged the integrity of intestinal mucous membrane barrier. Conclusion Intestinal mucosal barrier is injured by BMP2/4 via activation of NFκB signals after ischemic reperfusion.