Objective To analyze the expression and significance of Morinda officinalis water extract on Livin and Smac in testicular tissue of varicocele (VC) rats.Methods Selected 30 male SD rats and divided them into the sham operation group

the model group

and the observation group

with 10 rats in each group. VC model was constructed in the model group and the observation group

and no ligation was performed in the sham operation group. Normal saline was used in the sham operation group

normal saline was given to the model group after modeling

and the observation group was given the Morinda officinalis water extract after modeling. The indexes of testicles and epididymis were measured after operation. Extracted the left testicle to detect the expression of Livin and Smac

and to detect the cell apoptosis.Results There was no significant difference in body weigh among the three groups before and after modeling (P>0.05). The testicular index and epididymal index in the observation group were significantly higher than those in the model group

and the difference was statistically significant (P<0.05); but there was no statistically significant difference between the observation group and the sham operation group (P>0.05). The apoptosis index of testicular tissue in the observation group was significantly lower than that of the model group

and the difference was statistically significant (P<0.05); the model group was significantly higher than that of the sham operation group

and the difference was statistically significant (P<0.05). The positive cells rate and average optical density of Livin in testicular tissue in the observation group were significantly higher than those in the model group

and they were significantly lower than those in the sham operation group

with statistically significant differences (P<0.05).The rate of positive cells and average optical density of Smac in testicular tissue in the observation group were significantly lower than those in the model group

and they were significantly lower than those in the sham operation group

with statistically significant differences (P<0.05). Conclusion The Livin and Smac expressions in testicular tissue of varicocele SD rats could be effectively improved after the intervention of the Morinda officinalis water extract.