Objective To investigate the effects of Zingiber officinale on proliferation

apoptosis

invasion and migration of human oral squamous cell carcinoma. Methods The human oral squamous cell carcinoma cell lines were divided into blank group

5 μmol/L group

10 μmol/Lgroup and 20 μmol/L group.The blank group was cultured with conventional cell culture medium

and the other three groups were cultured with the corresponding concentration of Zingiber officinale.The proliferation

apoptosis

invasion and migration of the four groups were observed

and the expression levels of PI3K

protein kinase B (Akt) and Bcell lymphoma-2 (Bcl-2) were observed.Results The cell proliferation rates of 5 μmol/L group

10 μmol/L group and 20 μmol/L group were lower than that of blank group

and the cell proliferation rate of 20 μmol/L group was lower than those of 5 μmol/L and 10 μmol/L groups

the differences were significant(P<0.05).The apoptotic rates of 5 μmol/Lthat of blank group

and the apoptotic rate of 20 μmol/L group was higher than those of 5 μmol/L group and 10 μmol/L group

the differences were significant(P<0.05).The number of cell migration and invasion in 5 μmol/L group

10 μmol/L group and 20 μmol/L group were lower than those in blank group

and the number of cell migration and invasion in 20 μmol/L group was lower than those in 5 μmol/L group and 10 μmol/L group

the differences were significant (P<0.05).The expression levels of PI3K

Akt and Bcl-2 were all downregulated under the intervention of Zingiber officinale

and with the increase of Zingiber officinale concentration

the expression levels of PI3K

Akt and Bcl-2 were downregulated gradually

the differences were significant(P<0.05).Conclusion Gingerone can promote the apoptosis of human oral squamous cell carcinoma cells

inhibit the proliferation

migration and invasion of human oral squamous cell carcinoma cells

and its ability is concentrationdependent.