OBJECTIVE： To establish a method for the content determination of polymer in cefatrizine propylene glycol. METHODS： High performance sephadex gel chromatography was performed on the column of Sephadex G-10 with mobile phase A of 0.01 mol/L phosphate buffer [0.01 mol/L Disodium hydrogen phosphate solution-0.01 mol/L Sodium dihydrogen phosphate solution（61 ∶ 39，V/V）]（pH7.0） and mobile phase B of water at a flow rate of 1.0 ml/min， the detection wavelength was 254 nm， column temperature was 30 ℃， and volume injection was 200 μl. RESULTS： The linear range of polymer was 2.07-103.30 mg/ml（r＝0.999 4）； the limit of quantitation of 10.4 ng， limit of detection was 4.1 ng； RSDs of precision and reprodicibility tests were lower than 3％. CONCLUSIONS： The method is specific with high sensitivity and good reproducibility， and can be used for the content determination of polymer in active pharmacentical ingredient cefatrizine propylene glycol.