OBJECTIVE： To prepare Puerarin polymeric micelles and establish a method to determine its entrapment efficiency. METHODS： Puerarin polymeric micelles were prepared by film dispersion method. The polymeric micelles and free drug were separated by centrifugal-millipore filter filtration method. The entrapment efficiency of puerarin polymeric micelles was determined by HPLC. Diamonsil C18（2） column was used with 1％ citric acid solution-methanol （65 ∶ 35） at the flow rate of 1 ml/min. The detection wavelength was set at 250 nm， and column temperature was room temperature. RESULTS： The prepared polymeric micelles were spherical and spherical-like in shape with a mean particle size of 54.12 nm， polydispersity index of 0.122， Zeta potential of －13.60 mV； the linear range of puerarin was 2-10 μg/ml （R2＝0.999 4） with average recovery rate of 99.2％ （RSD＝0.9％， n＝3）. The recovery rate of free drug was 95.3％ （RSD＝1.7％， n＝3）. The mean entrapment efficiency and drug-loading amount of puerarin were（35.5±2.12）％ and （0.3±0.07）％， respectively （n＝3）. CONCLUSIONS： Film dispersion method is suitable for the preparation of Puerarin polymeric micelles. Established method is convenient， accurate and reliable for the content and entrapment efficiency determination of Puerarin polymeric micelles.