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目的: 建立蒙药大栀子超微饮片的质量标准。方法: 观察超微饮片的显微鉴别特征;采用薄层色谱法(TLC)对超微饮片进行定性鉴别;检测超微饮片水分、灰分、浸出物量;采用高效液相色谱法测定超微饮片中栀子苷的含量:色谱柱为Kromasil C18,流动相为乙腈-水(15 ∶ 85,V/V),流速为1.0 ml/min, 检测波长为238 nm,柱温为30 ℃,进样量为10 μl。结果:超微饮片细胞已完全破壁,粒径为5~60 μm。超微饮片TLC斑点清晰,分离良好。超微饮片水分为3.89%~5.55%,灰分为3.13%~5.22%,酸不溶性灰分为0.16%~0.80%,浸出物为29.2%~43.4%。栀子苷检测质量浓度线性范围为1.56~7.82 μg/ml(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为100.17%~102.04%(RSD=1.07%,n=6)。结论:该研究所建标准可用于蒙药大栀子超微饮片的质量控制。
OBJECTIVE: To establish the quality standard of ultramicro decoction piece of Meng medicine Gardenia jasminoides. METHODS: Microscopic identification character of the ultramicro decoction piece was observed; TLC was adopted for its qualitative identification; moisture, ash, extract were detected; HPLC was used for the content determination of geniposide: the column was Kromasil C18 with mobile phase of acetonitrile-water (15 ∶ 85,V/V) at a flow rate of 1.0 ml/min, detection wavelength was 238 nm, column temperature was 30 ℃, and injection volume was 10 μl. RESULTS: Cell wall was completely broken and the particle sizes were 5-60 μm. TLC spots were clear and well separated. The moisture was 3.89%-5.55%, ash was 3.13%-5.22%, acid-insoluble ash was 0.16%-0.80%, and extract was 29.2%-43.4%. The linear range of geniposide was 1.56-7.82 μg/ml (r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2.0%; recovery was 100.17%-102.04% (RSD=1.07%,n=6). CONCLUSIONS: The standard can be used for the quality control of ultramicro decoction piece of Meng medicine G. jasminoides.
蒙药大栀子超微饮片栀子苷质量标准
Gardenia jasminoidesUltramicro decoction pieceGeniposideQuality standard
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