OBJECTIVE： To establish a method for the contents determination of gallic acid， naringin， hesperidin， neohesperidin and baicalin in Qinghou liyan granule. METHODS： UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile - 0.1％ phosphoric acid （gradient elution） at a flow rate of 0.40 ml/min， detection wavelength was 280 nm， column temperature was 30 ℃， and injection volume was 1 μl. RESULTS： The linear range was 0.048-0.480 μg for gallic acid （r＝0.999 3）， 0.012-0.120 μg for naringin （r＝0.999 9）， 0.016-0.160 μg for hesperidin （r＝0.999 8）， 0.022-0.220 μg for neohesperidin （r＝0.999 9） and 0.072-0.720 μg for baicalin （r＝0.999 2）； limit of quantitation was 2.4，1.6，1.8，1.8，1.6 ng， limit of detection was 7.5， 5.0， 5.6， 5.6， 5.0 ng； RSDs of precision， stability and reproducibility tests were no lower than 3.0％； recoveries were 96.64％-102.02％ （RSD＝2.00％，n＝6）， 95.86％-102.56％ （RSD＝2.86％，n＝6），97.24％-102.54％（RSD＝2.10％，n＝6）， 97.44％-102.60％（RSD＝2.40％，n＝6） and 96.91％-103.13％ （RSD＝2.62％，n＝6）. CONCLUSIONS： The method is rapid and accurate， and can be used for the simultaneous determination of gallic acid， naringin， hesperidin， neohesperidin and baicalin in Qinghou liyan granule.