OBJECTIVE： To establish a method for simultaneous determination of gallic acid， chlorogenic acid， paeoniflorin and paeonol in Fule granules. METHODS： HPLC method was adopted. The determination was performed on Ultimate XB-C18 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were 270 nm（gallic acid，paeonol）， 325 nm （chlorogenic acid） and 230 nm （paeoniflorin）. The column temperature was 30 ℃， and sample size was 10 μL. RESULTS： The linear ranges of gallic acid， chlorogenic acid， paeoniflorin and paeonol were 0.076 2-1.524 μg（r＝0.999 7）， 0.037 6-0.751 μg （r＝0.999 9）， 0.030 3-0.606 μg （r＝0.999 7）， 0.020 6-0.412 μg（r＝0.999 8）， respectively. The limits of quantification were 0.353， 0.276， 0.421， 0.540 μg/mL， and the limits of detection were 0.121， 0.104， 0.148， 0.186 μg/mL. RSDs of precision， stability and reproducibility tests were all no more than 2.04％. The recoveries were 95.24％-100.47％（RSD＝1.59％，n＝9），99.49％-103.70％（RSD＝2.27％， n＝9）， 96.27％-101.09％（RSD＝1.94％，n＝9）， 95.05％-98.89％（RSD＝1.22％，n＝9），  respectively. CONCLUSIONS： The method is simple， accurate and reproducible， and can be used for the simultaneous determination of gallic acid， chlorogenic acid， paeoniflorin and paeonol in Fule granules.