OBJECTIVE： To optimize the ethanol extraction process of anti-atherosclerosis effective fraction of Lindley eupatorium. METHODS： Using the contents of hyperin， quercetin， kaempferol and jaceosidin as indexes， the effects of ethanol volume fraction， liquid-solid ratio， extraction time and extraction times on ethanol extraction technology were investigated. By multiple index scoring method， orthogonal test was designed to optimize extraction technology， and validation test was conducted. RESULTS： The optimal extraction technology was 70％ ethanol as extraction solvent with solid-liquid ratio of 12 ∶ 1， reflux extracting for 3 times， 2 h each time. Results of 3 batches of validation tests showed that the contents of 4 marker components were in high level， being 5.305 3， 1.560 1， 1.986 5， 6.703 6 mg/g in average （RSD＜1.1％，n＝3）. Results of validation tests  were stable and reliable， and were in good agreement with the results of orthogonal tests. CONCLUSIONS： The optimized ethanol reflux extraction technology of L. eupatorium is stable and reliable， and performs well. Energy consumption and cost are suitable for industrial production. The results provide technology basis for further purification and enrichment of anti-atherosclerosis effective components of L. eupatorium.