Objective：To investigate the prevalence and characteristics of infectious diarrhea caused by diarrheagenic Escherichia coli（E. coli） from 2015 to 2019 in a district of Shanghai，and to provide reference for prevention and control of infectious diarrhea. Methods：The surveillance data of the newly diagnosed suspected bacterial diarrhea in a tertiary hospital in Qingpu District of Shanghai from 2015 to 2019 were collected，and the epidemiological characteristics were analyzed by descriptive epidemiological method. Results：From 2015 to 2019，among the 4 494 patients’ stool or anal swab specimens，513 cases of diarrheagenic E. coli were detected，with the detection rate of 11.42%，and the mainly was enterotoxigenic E. coli（66.08%，339 cases），followed by enteroaggregative E. coli（18.91%，97 cases） and enteropa-thogenic E. coli（14.42%，74 cases）. The detection rate of diarrheagenic E. coli from 2015 to 2019 respectively was 9.10%，11.35%，16.57%，10.30% and 10.67%，and the differences among years were statistically significant（ ?字2=116.962，P<0.001）. The infectious diarrhea caused by diarrheagenic E. coli was commonly to see in summer and autumn. The main diarrhea symptom was watery stool（87.13%，447 cases），and some were accompanied with abdominal pain，nausea，vomiting or fever，among which 86.16% was single infection. The fever rate and abdominal pain rate of composite infection group were lower than those of single infection group，with statistical significance（fever： ?字2=4.936，P=0.026；abdominal pain： ?字2=17.079，P＜0.001）. There was significant difference in the detection rate among different age groups（ ?字2=40.672，P<0.001），with the highest detection rate in 21-30-year-old group（15.97%）. The detection rate of cadre staff（17.48%），medical staff（18.18%） and teachers（18.60%） was relatively high，with significant differences among the detection rate among different occupations（ ?字2=23.554，P=0.001）. Conclusion：The detection rate of diarrheagenic E. coli in Qingpu is quite high，mainly enterotoxigenic E. coli，showing local epidemic characteristics，mainly found in workers，and the differences among different ages and occu－pations are statistical significant. To survey its prevalence and characteristics can effectively provide scientific data for the prevention and control.