Objective：Through combined analysis of CA125 and HE4 levels to assess its diagnosis and prognosis prediction value and association with progression-free interval in patients with epithelial ovarian cancer Ⅱ. Methods：One hundred and one patients with epithelial ovarian cancer and 100 patients with ovarian epithelial benign tumors from April 2012 to October 2014 were taken as the subjects. The detection of CA125 and HE4 and the calculation of ROMA were performed to analyze the relationship between diagnosis and prognosis. Results：The levels of CA125 and HE4 in the benign tumors group and early and advanced ovarian cancer were statis－tically different from each other before treatment（P=0.000）. The sensitivity，specificity，and negative predictive value were the highest in the diagnosis of ovarian cancer，the misdiagnosis rate and the missed diagnosis rate were the lowest，and the correct diagnosis index was the highest；CA125，HE4 and ROMA had a higher diagnostic efficiency for epithelial ovarian cancer Ⅱ，especially ROMA values. Patients with CA125＞1 200 U/mL and HE4 ＞670 Pmol/L before treatment had a short recurrence time，and a shorter progression-free interval. There was statistic difference（CA125： χ2=20.926，P=0.000；HE4： χ2=11.235，P=0.001）. Conclusion：The combined diagnosis of CA125 combined with HE4 is superior in the diagnosis of epithelial ovarian cancer Ⅱ. Its preoperative level is negatively correlated with the progression-free interval of ovarian cancer patients，and it can assist the evaluation of prognosis. It is worthy of promotion and application in clinical work.