Objective：To compare the analgesic effect of positive psychological suggestion and dolantin injection in in vitro fertiliza－tion-embryo transfer（IVF-ET） via vaginal puncture. Methods：A total of 1 307 patients，with age less than 37 years old and without communication barrier and drug allergy history，who experienced controlled ovarian hyperstimulation in luteal phase throuth the short-term-effect long strategy and had 5-10 ovarian follicles，and experienced the IVF-ET in our department in 2018 were selected and divided into the dolantin group of 680 patients（intramuscularly injecting dolantin of 50mg before 30 minutes of operation） and the psychological suggestion group of 627 patients（positive psychological suggestion before 30 minutes of operation）. Pre-operative anxiety score，intraoperative pain and post-operative adverse reactions were compared between two groups. Results：The anxiety score in the psychological suggestive group was lower than that in the dolantin group，with statistically significant difference（t=3.462，P=0.000），and there was no significant difference in pain feeling between two groups（ χ2=0.601，P=0.438）. Post-operative blood pressure in the psychological suggestion group was not decreased，but it was significantly decreased in the dolantin group（t=-5.059，P=0.000）. The incidence rate of dizziness，nausea and vomiting in the psychological suggestion group was lower than that in the dolantin group，with staitistically significant difference（χ2=1181.762，P=0.000）. Conclusion：Before the IVF-ET to give positive psychological suggestion to patients can reduce anxiety，obtain same analgesic effect with dolantin，and effectively avoid adverse drug reactions caused by dolantin.