Objective：To investigate the clinical significance of low grade squamous intraepithelial lesions（LSIL） in different cell morphology by applying cervical cytology. Methods：Results of 153 patients diagnosed with LSIL by cervical cytology and underwent human papilomavirus（HPV） typing detection，colposcopy and cervical biopsy from July 2017 to June 2018 were retrospectively ana－lyzed. Results：The LSIL patients with different cell morphology diagnosed by cervical cytology had high infection rates of high-risk HPV（HR-HPV）（81.0%）；the infection rate of LSIL patients with koilocyte was 77.0%，while of LSIL patients with non-koilocytotic cell was 83.7%. Among them，the HPV16 and HPV18 infection rates of LSIL patients with abnormal cells being non-koilocytotic cell were 18.5%（17/92），significantly higher than those of koilocyte type LSIL patients（6.6%，4/61）. The detection rate of biopsy patho－logical diagnosis ≥ intraepithelial lesion of cervix（CIN）Ⅱ among LSIL patients with non-koilocytotic cells was 28.3%（26/92），sig－nificantly higher than that among LSIL patients with koilocyte type（8.2%，5/61），with were statistically significant differences（P<0.05）. Conclusion：The LSIL patients with non-koilocytotic cells are more likely to have or progress into CIN Ⅱ and more severe lesions，therefore，clinicians should pay more attention to it.