Objective：To evaluate the effect of transvaginal bilateral sacrospinous ligament suspensions fixation on treating anterior and middle pelvic organ prolapse. Methods：Fifty-seven patients with pelvic prolapse of degree Ⅱ or above who had clinical symptoms and surgical intention were selected and were underwent transvaginal bilateral sacrospinous ligament suspensions fixation. Patients’ age，operation time，bleeding volume，and pre- and post-operation POP-Q score and PFDI-20 score were recorded and were analyzed. Results：The average operation time was （57.70±21.42） min and the average blood loss was （45.44±40.00） mL. Scores of Aa，Ba，C and PFDI-20 before and after surgery had statistically significant differences（P<0.05）. Conclusion：Transvaginal anterior sacrospinous ligament suspensions fixation is a safe and effective method for treating patients with anterior and middle pelvic organ prolapse.