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西南医科大学厅市共建中枢神经系统药物四川省重点实验室
纸质出版日期:2021,
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[1]王元园,赵文美,李柯,曽明唐,皮超,魏郁梦,赵领.龙眼主要活性成分分析方法建立及应用[J].西南医科大学学报,2021,44(02):114-119.
WANG Yuanyuan, ZHAO Wenmei, LI Ke, et al. Establishment and application of an analytical method for the main active ingredients in longan[J]. Journal of Southwest Medical University, 2021,44(2):114-119.
目的:建立简便、准确的龙眼主要成分分析方法。方法:以紫外分光光度法建立各成分分析方法
并利用该方法测定泸产养生酒中总糖、总黄酮和多酚的含量。结果:利用紫外分光光度计成功建立了龙眼主要活性成分分析方法。几种主要成分回归方程的R2均大于0.999
表明各方法线性拟合的方法可靠
精密度的RSD值均小于2%
回收率均在80%~120%之间
符合分析方法的要求。并且
利用该方法成功测定龙眼养生酒中各主要成分的含量。其中
养生酒Y-3、Y-4、Y-5、Y-6总糖含量分别为20.94 mg/mL、49.01 mg/mL、39.70 mg/mL、47.61 mg/mL。养生酒Y-3、Y-4、Y-5、Y-6多酚含量分别为0.597 mg/mL、0.291 mg/mL、0.473 mg/mL、0.555 mg/mL。养生酒Y-3、Y-4、Y-5、Y-6总黄酮含量分别为69.58μg/mL、50.41μg/mL、292.92μg/mL、387.45μg/mL。结论:该龙眼主要成分分析方法可用于含龙眼主要成分物质的测定。
Objective: To establish a simple and accurate method for the analysis of main active components in longan. Methods: An analytical method based on ultraviolet spectrophotometry was established to measure the content of total sugar
total flavonoids
and total polyphenols in Luzhou longan wine. Results: An analytical method for the main active ingredients in longan was successfully established based on ultraviolet spectrophotometer. The regression equations of major components had R
2
values greater than 0.999
indicating that the linear fitting of each component was reliable. The precision values expressed as relative standard deviation were less than 2%
and the recoveries were 80%~120%
which met the requirements of analysis. The content of main ingredients in longan wine was successfully determined by this method. The total sugar content of Y-3
Y-4
Y-5
and Y-6 was 20.94
49.01
39.70
and 47.61 mg/mL
respectively. The total polyphenol content of Y-3
Y-4
Y-5
and Y-6 was 0.597
0.291
0.473
and 0.555 mg/mL
respectively. The total flavonoid content of Y-3
Y-4
Y-5
and Y-6 was 69.58
50.41
292.92
and 387.45 μg/mL
respectively. Conclusion: This method can be used for determination of the main components in longan.
龙眼分析方法紫外分光光度法
LonganAnalytical methodUltraviolet spectrophotometry
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