Objective:To investigate the solubilization effect of β-cyclodextrin（β-CD）on ciclopirox olamine（CPX）using the β-CD inclusion technology

and to improve the solubility and absorption of CPX

optimize the clinical preparation of CPX

promote drug absorption by the skin

and improve the bioavailability of such drugs.Methods:The ultrasonic method was used to prepare CPX-β-CD inclusion complex

and an infrared spectrometer and a scanning electron microscope were used for phase identification.High-performance liquid chromatography was used to measure the content and calculate inclusion rate and release rate

and in vitro release assay was used to observe the effect of β-CD inclusion complex on the drug release of CPX.Results:The optimal chromatographic conditions were a flow rate of 1.0 mL/min

a detection wavelength of 304 nm

a column temperature of 30℃

active compound 200 μg/mL

an injection volume of 5 μL

and a mobile phase of acetonitrile and 0.1% EDTA-2 Na（v/v

45∶55）.The detection concentration of CPX showed a good linear relationship within the range of 20.0 ～ 200.0 μg/mL（R2= 0.9998）.Relative standard deviation was < 2% for precision

repeatability

stability

and recovery.At a ratio of 1∶2 for CPX and β-CD

the inclusion complex prepared by the ultrasonic method had the highest inclusion rate of 47.72%.The inclusion complex had a higher drug release amount than the bulk drug of CPX at each time point

suggesting that the inclusion action can improve the release efficiency of CPX.Conclusion:CPX-β-CD inclusion complex is successfully prepared in this experiment

and the preparation process is simple and feasible.The prepared CPX-β-CD inclusion complex has high stability and can effectively improve the release efficiency of CPX.