TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响

李爱迪; 聂敏海; 左东川; 饶勇; 曾锦

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TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响

基础医学研究 | 更新时间：2023-04-14

- TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响
- Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts
- 西南医科大学学报 2020年43卷第2期页码：135-139
- 作者机构：
  
  1. 西南医科大学附属口腔医院牙周黍膜科
  2. 西南医科大学心血管医学研究所
  3. 附属口腔医院正畸科
- 作者简介：
- 基金信息：
  
  四川省科技厅项目（2018JY0401）;西南医科大学附属口腔医院基金（0800103009）;西南医科大校级青年资金（2017-ZRZD-001）
- DOI：
  中图分类号： R329.2
- 纸质出版日期：2020，
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[1]李爱迪,聂敏海,左东川,饶勇,曾锦.TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J].西南医科大学学报,2020,43(02):135-139.

LI Aidi, NIE Minhai, ZUO Dongchuan, et al. Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts[J]. Journal of Southwest Medical University, 2020,43(2):135-139.
[1]李爱迪,聂敏海,左东川,饶勇,曾锦.TRPM7通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J].西南医科大学学报,2020,43(02):135-139. DOI：

LI Aidi, NIE Minhai, ZUO Dongchuan, et al. Effect of transient receptor potential melastatin 7 channel on the proliferation and migration of human buccal mucosal fibroblasts[J]. Journal of Southwest Medical University, 2020,43(2):135-139. DOI：

摘要

目的:探讨瞬时受体电位离子通道TRPM7 （transient receptor potential melastatin-7）在人口腔颊黏膜成纤维细胞增殖和迁移中的作用。方法:逆转录聚合酶链式反应、免疫荧光法及全细胞膜片钳检测成纤维细胞中TRPM7的表达。MTT和细胞划痕实验分别检测应用2-氨基乙基联苯基硼酸酯（2-APB）或shRNA抑制TRPM7通道对成纤维细胞增殖和迁移的影响。结果:人口腔颊黏膜成纤维细胞功能性表达TRPM7通道。TRPM7 shRNA作用48 h后成功阻断TRPM7通道

500μmol/L2-APB可抑制其表达

两组与对照组相比

膜片钳技术记录到TRPM7样电流明显减弱

在24 h后均明显抑制成纤维细胞的增殖和迁移能力

差异有统计学意义（P <0.05）。结论:TRPM7通道参与人口腔颊黏膜成纤维细胞增殖以及迁移能力的调控。

Abstract

Objective: To investigate the role of transient receptor potential melastatin 7（TRPM7） in the prolifer-ation and migration of human buccal mucosal fibroblasts. Methods: Reverse transcription-polymerase chain reac-tion

immunofluorescence method

and whole-cell patch clamp were used to determine the expression of TRPM7 infibroblasts. MTT and wound-healing assays were used to examine the effects of inhibiting TRPM7 channels using2-aminoethyl diphenylborinate（2-APB） or shRNA on fibroblast proliferation and migration. Results: TRPM7 chan-nels were functionally expressed in human buccal mucosal fibroblasts. TRPM7 channels were successfully blockedafter 48 h of TRPM7 shRNA treatment

and their expression was inhibited by 500 μmol/L of 2-APB. Comparedwith the control group

the TRPM7-like currents were significantly decreased in the two experimental groups accord-ing to patch-clamp recording

and the proliferation and migration abilities of fibroblasts were significantly sup-pressed after 24 h of treatments（P < 0.05）. Conclusion: The TRPM7 channel is involved in the regulation of prolif-eration and migration of human buccal mucosal fibroblasts.

关键词

成纤维细胞TRPM7细胞增殖细胞迁移

Keywords

FibroblastsTransient receptor potential melastatin 7Cell proliferationCell migration

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