Objective To investigate the expression of Survivin and Proliferating Cell Nuclear Antigen（Ki-67） in breast cancer and its significance.Method 60cases of specimens of breast cancer tissues were detected the expression of Survivin and Ki-67in these tissues with the immunohistochemical method

while 60cases of para-carcinoma tissues were selected as comparison.Results Cases of Survivin positive in the breast cancer tissues were 44

accounting for 73.3%

while cases of Survivin positive in the para-carcinoma tissues were 4

accounting for 10.0%.Cases of Ki-67positive in the breast cancer tissues were 40

accounting for 66.7% and cases of Ki-67positive in the para-carcinoma tissues were 3

accounting for 5.0%.The expression of Survivin and Ki-67in the breast cancer tissues was higher than that of the para-carcinoma tissues（P<0.05）.The expression of Survivin positive was not related with the size or the differentiation of tumor but with lymphatic metastasis（P<0.05）;the expression of Ki-67positive was not related with the size of tumor but with its differentiation and lymphatic metastasis（P<0.05）.Conclusion The expression of Survivin and Ki-67in breast cancer has important clinical significance

closely related to the cause and development of breast cancer.It can be used as one of the reference index to evaluate the prognosis and efficacy of breast cancer.