Objective To study the effects of the transfection of antisense human tankyrase 1 on the morphology of SGC-7901.Method Antisense tankyrase 1 eukaryotic expression on vector pcDNA3.1 was constructed with recombinant DNA technique and transfected into SGC-7901 with liposome DOTAP.Morphological changes of the transfected and non-transfected cells were examined under light and electron microscopy.Results Under light microscopy

transfected cells with antisense gene to tankyrase 1 were larger with abundant cytoplasm and had less nuclei division.The ratio of nuclei to cytoplasm decreased as compared with their parent cells.Under electron microscopy

organelles in these cells were abundant and nuclear membrane was more regular.Conclusion Tankyrase 1 antisense gene can partially reverse malignant phenotypes of SGC-7901 cells and promote its differentiation.