Objective       To determine the effect of liver X receptor (LXR) specific agonist

GW3965 on the expression of B lymphocyte stimulator (BLyS) in human B-cell lymphoma Raji cells.     Methods    Cell viability of Raji cells exposure to GW3965 at concentrations of 0.5

5 and 10 μmol/L for 24 h was detected by MTT assay. Then the expression of ATP-binding cassette (ABCA1

the specific target gene of LXR) mRNA and LXR mRNA was detected by RT-PCR and the expression of BLyS at mRNA and protein levels were measured by RT-PCR and Western blotting.     Results    The level of 1‰ DMSO OD(492 nm)  was（0.632±0.055）

and 0.5

5 and 10 μmol/L GW3965 resulted in the levels of OD(492nm) were （0.609±0.073）

（0.612±0.052） and（0.628±0.055） respectively

indicating that GW3965 had no effect on the viability of Raji cells (P0.05). Exposure to 0.5 and 5 μmol/L GW3965 results in an increase in the level of ABCA1 mRNA in a dose-dependent manner

suggesting LXR was functional in Raji cells. The expression of BLyS were down-regulated in both mRNA and protein levels after LXR activation.     Conclusion    LXR decreases the expression of BLyS in Raji cells.