Xue Xia,Shi Juanzi,Zhang Silin,Zhao Wanqiu.Impact of human pronuclear stage score on pregnancy rates[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(15):1670-1672.
Xue Xia,Shi Juanzi,Zhang Silin,Zhao Wanqiu.Impact of human pronuclear stage score on pregnancy rates[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(15):1670-1672.DOI：
目的 探讨多个原核期形态学指标与取卵后第3天（D3）胚胎移植后妊娠结局之间的关系，筛选能反映胚胎移植后种植潜能的原核期形态指标。 方法 回顾性分析本院生殖中心2007年11月至2008年2月的186例行常规体外受精/单精子卵胞浆内注射胚胎移植的不孕症患者，共395个胚胎，选取年龄≤35岁，第3天进行胚胎移植后妊娠的共84例患者为妊娠组；没有妊娠的不孕症患者共102例为未妊娠组。根据原核期形态包括两原核是否相邻、居中、大小、排列；核仁前体（NPB）的排列方式、大小、在原核中的位置；极体和原核长、纵轴夹角，进行数据统计，比较原核期形态和胚胎妊娠之间的关系。 结果 妊娠组与未妊娠组相比，两原核形态、两NPB形态、两极体方位均有统计学差异（P0.01）；妊娠组两原核形态A级明显高于B、C、D级（67.03% vs 20.88%、5.49%、6.59%，P0.01）；妊娠组两NPB形态1级和2级明显高于3、4级（38.46%、41.21% vs 17.03%、3.30%，P0.01），妊娠组两极体方位α和β明显高于γ(48.35%、36.81% vs 14.84%
P0.05)。 结论 原核期形态可以反映受精卵的质量，可以为临床选择移植胚胎提供依据。
Abstract
Objective To study the relation between pronuclear stage morphologic indexes of zygotes and pregnancy rates and to screen the pronuclear stage morphologic indexes of zygotes indicating the embryo pregnancy potential in 3 d after in vitro fertilization and embryo transfer (IVF-ET). Methods One hundred and eighty-six patients at the age of below 35 years after IVF-ET in our center from Nov. 2007 to Feb. 2008 were enrolled in this study. The patients undergoing embryo transfer on day 3 after oocyte retrieval were divided into pregnancy group (n=84) and pregnancy failure group as control group (n=102). The morphologic characteristics of zygotes were analyzed including pronuclei
nucleolar precursor bodies (NPB)
polar body (PB) located in the longitudinal or perpendicular axis of pronuclei and compared between the two groups. Results In the pregnancy group
the most frequent configuration was that with centralized
juxtaposed pronuclei (pattern A
67.03%) compared to all the others(pattern B
20.88%; pattern C
5.49%; pattern D，6.59%
P0.01）； the most proportion of nuclear precursor bodies morphology was represented by largesized nuclei aligned or scattered in both pronuclei were observed in patterns 1 and 2(38.46% and 41.21%) compared with patterns 3 and 4(17.03% and 3.30%，P0.01); the most frequent configuration was represented by that where polar bodies were located in the longitudinal and perpendicularly axis of pronuclei(pattern α，48.35%；pattern β 36.81%)compared with others patternsγ(14.84%
P0.05). Conclusion The morphology of two pronuclei can reflect the quality of zygotes and may help predict the developmental potential of embryo chosen on day 3 after IVF.
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