Objective  To investigate the origin of bacteria in sterile packages for war readiness under natural environment in inland region. Methods  Genomic DNAs from the Bacillus cereus

Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages for war readiness and from air bacteria strains in war readiness warehouse were extracted

and then used as templates to amplify the 16S rRNA and 23S rRNA ITS (internal transcribed spacer) sequences by PCR. The PCR products were extracted and sequenced

and the sequences homology analysis was performed with BLAST. Results  Amplification products of DNA PCR from Bacillus cereus

Micrococcus luteus and Staphylococcus cohnii ureal were about 800

1 200 and 1 000 bp respectively; The 16S rRNA-23S rRNA ITS sequences of bacterial strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse. Conclusion  The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in inland region.