Objective    To investigate the effect of zinc finger protein A20 expression on lipopolysaccharide (LPS) response in human monocytes so as to discuss its regulation roles and mechanism to inflammation in vivo and its potential value of clinical application.     Methods    Cell transfection technique was respectively used to make zinc finger protein A20 transgenic cell model and gene silence cell model

both of which were then treated with LPS. Real-time reverse PCR was employed to analyze the expression of zinc finger protein A20 and ELISA to determine the activity of nuclear transcription factor NF-κB and the levels of dependent inflammatory cytokines including tumor necrosis factor (TNF) alpha.     Results    After LPS treatment

the NF-κB activity and the TNF-alpha level were significantly lower in the cells with overexpressed zinc finger protein A20 than LPS control cells (P0.01)

but significantly higher in zinc finger protein A20 gene silenced cells (P0.01).     Conclusion    Zinc finger protein A20 can effectively regulate LPS responses in human monocytes and markedly abate expressions of important nuclear factors and proinflammatory cytokines

proving that zinc finger protein A20 has potential clinical value for over inflammatory reaction of sepsis.