食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析

汪斌; 李俊材; 傅仲学; 朱旦

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食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析

论著 | 更新时间：2026-01-09

- 食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析
- Proteomic analysis of lymph nodes affected by esophageal squamous cell carcinoma by two-dimensional gel electrophoresis
- 陆军军医大学学报 2007年29卷第4期页码：354-356
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  中图分类号： R322.25;R730.43;R735.1
- 纸质出版：2007
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汪斌,李俊材,傅仲学,朱旦.食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):354-356.

　WANG Bin,LI Jun-cai,FU Zhong-xu,ZHU Dan.Proteomic analysis of lymph nodes affected by esophageal squamous cell carcinoma by two-dimensional gel electrophoresis[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):354-356.
汪斌,李俊材,傅仲学,朱旦.食管鳞癌转移淋巴结蛋白质双向凝胶电泳图谱差异分析[J].陆军军医大学学报(原第三军医大学学报),2007,29(04):354-356. DOI：

　WANG Bin,LI Jun-cai,FU Zhong-xu,ZHU Dan.Proteomic analysis of lymph nodes affected by esophageal squamous cell carcinoma by two-dimensional gel electrophoresis[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(04):354-356. DOI：

摘要

目的分析食管鳞癌转移淋巴结与正常淋巴结组织蛋白质表达差异。方法采用双向凝胶电泳（2-DE）技术和计算机辅助的图像分析方法，对食管鳞癌转移淋巴结与正常淋巴结组织蛋白质进行分离和比较分析。结果获得了背景清晰、分辨率和重复性较好的双向凝胶电泳图谱，比较分析显示23个蛋白点的表达量存在明显差异，其中新增蛋白点4个，缺失1个，14个蛋白点表达发生明显上调，4个蛋白点表达发生明显下调。结论食管鳞癌转移淋巴结与正常淋巴结蛋白质表达存在明显差异，可能与食管鳞癌淋巴结转移机制有关。

Abstract

Objective To analyze the differential expression of proteome between metastatic lymph nodes and normal lymph nodes in esophageal squamous cell carcinoma. Methods Two dimensional polyacrylamide gel electrophoresis (2-DE) technique and computer-assisted image analysis were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes. Results There were 23 protein spots significantly differentially expressed

of which 4 protein spots were newly found

1 protein spot disappeared

14 protein spots increased markedly

and 4 protein spots decreased significantly. Conclusion The proteomic expression in metastatic lymph nodes of esophageal squamous cell carcinoma is significantly different from that of the normal lymph nodes

which may be associated with the mechanism of metastasis of esophageal squamous cell carcinoma.

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