人iASPP全长CDS的分段扩增、克隆及鉴定

陈杰; 辛海明; 蔡云; 侯露; 王刚; 刘利; 卢欣; 钟山; 刘泽军

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人iASPP全长CDS的分段扩增、克隆及鉴定

论著 | 更新时间：2026-01-09

- 人iASPP全长CDS的分段扩增、克隆及鉴定
- Cloning and identification of human iASPP full-length CDS region
- 陆军军医大学学报 2007年29卷第11期页码：996-998
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  中图分类号： R394-33;R394.3
- 纸质出版：2007
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陈杰,辛海明,蔡云,侯露,王刚,刘利,卢欣,钟山,刘泽军.人iASPP全长CDS的分段扩增、克隆及鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):996-998.

　CHEN Jie,XIN Hai-ming,CAI Yun,HOU Lu,WANG Gang,LIU Li,LU Xin,ZHONG Shan,LIU Ze-jun.Cloning and identification of human iASPP full-length CDS region[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):996-998.
陈杰,辛海明,蔡云,侯露,王刚,刘利,卢欣,钟山,刘泽军.人iASPP全长CDS的分段扩增、克隆及鉴定[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):996-998. DOI：

　CHEN Jie,XIN Hai-ming,CAI Yun,HOU Lu,WANG Gang,LIU Li,LU Xin,ZHONG Shan,LIU Ze-jun.Cloning and identification of human iASPP full-length CDS region[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(11):996-998. DOI：

摘要

目的最初报道的iASPP长度为351个氨基酸，但最近发现iASPP的全长应为828个氨基酸，为了探讨全长iASPP的功能，我们采取3分段扩增的策略克隆全长iASPP基因。方法从HL-60细胞中提取总RNA、DNA

RT-PCR分段扩增目的基因，克隆至pMD19-T simple载体，测序，并亚克隆至pCDNA3.1载体。结果重组真核表达质粒pcDNA3.1(+)-iASPP经测序，与GenBank上登陆的人iASPP cDNA（gi:60457962）序列完全一致。结论成功构建了iASPP真核表达载体。

Abstract

Objective iASPP was originally reported as long as 351 amino acids. Now

it is clear that the full-length of iASPP is as long as 828 amino acids. To investigate the function of full-length iASPP

we use three-step PCR technique to clone the full-length CDS of iASPP. Methods The total RNA was extracted from HL-60 and cDNA was amplified by RT-PCR. Then the amplified PCR product was digested and inserted into pMD19-T simple vector and subcloned into pCDNA3.1 vector. Results The gene sequence of iASPP was consistent with that reported (gi:60457962) in GenBank with cDNA sequencing. Conclusion The recombinant eukaryotic expression vector containing CDS region of iASPP has been constructed successfully.

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