3 new SCAR markers for Dendrobium williamsonii Day et Rchb. f.，Dendrobium findlayanum E. C. Parish et Rchb. f. and Pholidota chinensis Lindl. by improved RAPD amplification

doi:10.3969/j.issn.2096-3351.2024.01.007

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3 new SCAR markers for Dendrobium williamsonii Day et Rchb. f.，Dendrobium findlayanum E. C. Parish et Rchb. f. and Pholidota chinensis Lindl. by improved RAPD amplification

更新时间：2024-04-11

- 3 new SCAR markers for Dendrobium williamsonii Day et Rchb. f.，Dendrobium findlayanum E. C. Parish et Rchb. f. and Pholidota chinensis Lindl. by improved RAPD amplification
- Journal of Southwest Medical University Vol. 47, Issue 1, Pages: 29-33(2024)
- 作者机构：
  
  西南医科大学医学基础研究中心,泸州,646000
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.2096-3351.2024.01.007
  CLC：
- Published：2024，
  
  Published Online：20 January 2024，
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3 new SCAR markers for Dendrobium williamsonii Day et Rchb. f.，Dendrobium findlayanum E. C. Parish et Rchb. f. and Pholidota chinensis Lindl. by improved RAPD amplification. [J]. Journal of Southwest Medical University 47(1):29-33(2024)
DOI：

3 new SCAR markers for Dendrobium williamsonii Day et Rchb. f.，Dendrobium findlayanum E. C. Parish et Rchb. f. and Pholidota chinensis Lindl. by improved RAPD amplification. [J]. Journal of Southwest Medical University 47(1):29-33(2024) DOI： 10.3969/j.issn.2096-3351.2024.01.007.

摘要

目的寻找快速区分黑毛石斛、蜂腰石斛和石仙桃的序列特征扩增区域（sequence characterized amplified regions，SCAR）新标记。方法在改良的随机扩增多态性DNA （random amplified polymorphic DNA，RAPD）的基础上对10个物种进行基因组DNA随机扩增、分子克隆和测序，分别设计了可鉴别3个物种的SCAR标记。结果成功筛选出3 个SCAR标记，其相应的特异性标记分别为N2-12、A2-17和A6-33；其中SCAR标记N2-12在黑毛种属中扩增出约750 bp的特异性条带，SCAR标记A2-17在蜂腰石斛中扩增出约750 bp的特异性条带，SCAR标记A6-33在石仙桃种属中扩增出约600 bp的特异性条带。结论 N2-12、A2-17和A6-33这3对特异性SCAR新标记能快速、准确的区分出黑毛石斛、蜂腰石斛和石仙桃这3个物种，为今后进行该中药道地药材样品的鉴定提供了实验依据。

Abstract

Objective To find the SCAR（sequence characterized amplified regions） markers that can quickly distinguish Dendrobium williamsonii Day et Rchb. f.， Den. findlayanum Par. et Rchb. f. and Pholidota chinensis Lindl.. Methods In this paper，based on random amplified polymorphic DNA （RAPD），we randomly amplified the genomic DNA of 10 species，using the method of molecular cloning and sequencing，and got the SCAR markers which can identify the three species. Results Three specific markers （N2-12，A2-17，A6-33） were screened. The primer N2-12 amplified a specific band of about 750 bp in the species of Dendrobium williamsonii Day et Rchb. f.，A2-17 amplified a specific band of about 750 bp in the species of Den. findlayanum Par. et Rchb. f.，and A6-33 amplified a specific band of about 600 bp in the species of Pholidota chinensis Lindl.. Conclusion These three pairs of specific SCAR markers could quickly and accurately distinguish the above three species，providing an experimental basis for the identification of this authentic Chinese medicinal plant in the future.

关键词

黑毛石斛蜂腰石斛石仙桃序列特征扩增区域标记鉴定

Keywords

Dendrobium williamsonii Day et Rchb. f.Dendrobium findlayanum E. C. Parish et Rchb. f.Pholidota chinensis Lindl.SCAR markerIdentification

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