Objective To optimize the extraction process conditions of Shugan Lidan Granules，and preliminary evaluation of its liver protection effect，to provide a basis for the development of the preparation. Methods Orthogonal test method was used to evaluate the three factors of water addition，extraction time and extraction times based on the yield of dry paste，the transfer rate of emodin and chrysophanol，and the best water extraction process of Shugan Lidan Granules. Investigate the therapeutic effects of different doses of water extracts on alpha-naphthylisothiocyanate-induced cholestasis in mice，and clarify the efficacy of water extracts. Results The best water extraction process was to add 10 times the amount of water and extract three times for 1.5 h each time. Compared with the model group，7 days after administration，serum biochemical indicators and liver tissue inflammatory factor mRNA levels were significantly reduced，and the degree of liver tissue pathological damage was significantly reduced. Conclusion The optimal extraction process was stable and feasible，and the results of pharmacodynamic studies suggest that the water extract of Xiaoyan Lidan Recipe showed certain protective effects on the liver injury of cholestatic induced by ANIT in mice.