Objective To screen the optimal extraction conditions of total flavonoids from Cynodon dactylon. Methods The NaNO2-Al（NO3）3-NaOH colorimetry was used to determine the content of total flavonoids，and the standard curve method was used for content analysis. The content of total flavonoids was used as the index for screening the best extraction process. The extraction time，extraction temperature，ethanol volume and solid-liquid ratio of different gradients were investigated by single factor experiment. Four factors and three horizontal orthogonal experiments were used to optimize the extraction process conditions and screen the best extraction technology of total flavonoids from Cynodon dactylon. Results The optimal extraction method of total flavonoids from Cynodon dactylon was ethanol reflux extraction. The optimal extraction process was 80% ethanol，the material-liquid ratio was 1∶60 （g/mL），and the reflux extraction time was 1.5 h with 100°C extraction temperature. Conclusion This method in this experiment was stable and feasible，and could be used for the extraction of total flavonoids in Cynodon dactylon，which provided scientific basis for study of quality standard of this medicinal material.