Objective To check problems in the radiosynthesis of 68Ga-DOTATATE and restore the labeling yield of the reaction and the radiochemical purity of the final product. Methods When the labeling yield of 68Ga-DOTATATE synthesis was found abnormal，all relevant processes were checked，including the pH of reactive solution，buffer solution，precursor concentration，reaction time，and heating system. A radio-high-performance liq⁃uid chromatography was used to measure the labeling yield of 68Ga-DOTATATE synthesis under different conditions and determine the effects of individual parameters on the labeling yield. Results However，there were still abnor⁃mal RCYs occurred even with the shaking incubator. In the test of comparing results from a module and the shaking incubator

it was found unexpectantly that the RCY from the module was restored back to normal. Further investigat⁃ing our experimental results

we concluded that the problem might come from a mild contamination of the hydrochlo⁃ric acid. By executing strict usage guidelines for the Ge-Ga generator

the problem was resolved completely. The RCY of the reaction and the RCP of the final product were restored as 68Ga-DOTATATE was synthesized using a module.Conclusion This systematic investigation of problems in the radiosynthesis of 68Ga-DOTATATE provides a checking protocol to help identify problems，and also provides corresponding preventive measures to avoid repeated occurrence of similar problems.