ObjectiveTo evaluate the clinical effects of electro-acupuncture treatment to ICU-acquiredweakness（ICUAW）in sepsis patients with mechanism ventilation（MV）at early stage by bedside diaphragmatic ultrasound.MethodsSeventy-five sepsis patients with MV were enrolled and randomly divided into 3 groups based on random numberlist:early-stage intervention group（n=25）

late-stage intervention group（n=25）and control group（n=25）.Electro-acupuncturetreatment was performed within 24 h and 72 h in early-stage intervention group and late-stage intervention group

respectively.All the patients underwent medical research council scale（MRC）evaluation before weaning.The activity of the right diaphragm

bilateral diaphragmatic thickening fraction（DTF）and bilateral diaphragmatic excursion-time（E-T）index were measured bybedside diaphragmatic ultrasound on the 0（T0）

3 rd（T1）

and 7 th（T2）day after enrollment.ResultsThe MV time and ICUday in both early-stage intervention group and late-stage intervention group were lower than those in control group（all P<0.05）.The levels of MRC in early-stage intervention group was higher than that in control group and late-stage intervention group（bothP<0.05）.The levels of right diaphragm activity

right DFT

left DFT

right E-T and left E-T in early-stage intervention groupand late-stage intervention group were higher than those in control group on T2（all P<0.05）. The relation indexs betweenelectro-acupuncture treatment or early-stage electro-acupuncture treatment and diaphragm ultrasound were 0.249～0.598 and0.288～0.623

respectively. And both of them had the maximum correlation with right DTF（r=0.598

0.598

both P<0.001）.ConclusionBeside diaphragmatic ultrasound is helpful for the evaluation of protective effect on diaphragmatic function underelectro-acupuncture treatment in MV sepsis patients.