Objective To investigate the clinical application value of double contrast-enhanced ultrasound in evaluating the proliferation

invasion and angiogenesis of cancer cells in gastric cancer patients.Methods Forty patients with gastric cancer admitted in our hospital were selected as the observation group

and 50 healthy patients with simultaneous gastroscopy were taken as the control group.The enhancement time（ET）

time-to-peak（TTP）and wash-in-rate（WIR）were measured by double contrast-enhanced ultrasound

the expression of invasion gene and angiogenesis index of gastric cancer cells were compared

and the correlation between contrast-enhanced ultrasound parameters and invasion and angiogenesis index was analyzed.Results The levels of ET

TTP and E-cadherin in the observation group were significantly lower than those in the healthy control group

while WIR and the levels of eukaryotic initiation factor 4e（EIF4E）

CD44

ring finger domain protein 1（UHRF1）

endothelial growth factor（VEGF）

growth-promoting factor（IGF-1）

α-1-microglobulin-trypsin inhibitor precursor（AMBP）and soluble E-cadherin（SE-CAD）in the observation group were significantly higher than those in the healthy control group（all P<0.05）. Correlation analysis showed that ET and TTP were positively correlated with E-cadherin

and negatively correlated with EIF4E

CD44

UHRF1

VEGF

IGF-1

AMBP

SE-CAD（all P<0.001）

WIR was negatively correlated with E-cadherin

and positively correlated with EIF4E

CD44

UHRF1

VEGF

IGF-1

AMBP

SE-CAD（all P<0.001）.Conclusion Double contrast-enhanced ultrasound can accurately evaluate the invasion and angiogenesis of cancer cells in gastric cancer patients

which has good clinical application value.