Objective To prepare 10-hydroxycamptothecin（10-HCPT） loaded liquid fluorocarbon targetingnanoparticles

and to investigate their targeting ability to ovarian carcinoma cells（SKOV-3） and the effect of ultrasound/computed tomography（CT） dual-mode imaging in vitro.Methods By using hydroxy terminated lactic acid/glycolic acidcopolymer（PLGA-COOH）

perflurooctyl bromide（PFOB）and 10-HCPT

PLGA@10-HCPT-PFOB nanoparticles were preparedby double emulsion solvent evaporation method.The surface morphology and internal structure were observed

the particle size

surface potential

encapsulation efficiency and drug loading of 10-HCPT were measured as well. The cRGD peptide wasconjugated to the nanoparticles by carbodiimide technique. The binding of cRGD-PLGA@10-HCPT-PFOB was detected bylaser scanning confocal microscope and flow cytometer.The targeting properties of the nanoparticles to SKOV-3 were detected.The ability of ultrasound/CT imaging of targeted nanoparticles was evaluated in vitro.Results The targeted nanoparticles wereproduced as a yellow suspension.The size distribution of the nanoparticles was uniform observed by optical microscope

with amean diameter of（322.03±5.34）nm and surface potential was（-1.55±0.10）mV.The surface of the targeted nanoparticles wassmooth observed by the scanning electron microscope. The core-shell structure was observed by the transmission electron microscope

and the PFOB and 10-HCPT were trapped in PLGA.The drug encapsulation and drug loading capacity of 10-HCPTwere（81.34 ± 2.28）% and（13.24 ± 1.24）%

respectively. Laser scanning confocal microscope showed that the FITC-labeledcRGD peptides were green and it fused to orange with the red nanoparticles labeled DiI.The flow cytometer showed that theintensity of FITC in PLGA@10-HCPT-PFOB and cRGD-PLGA@10-HCPT-PFOB nanoparticles were 0.78% and 80.76%

respectively. The laser scanning confocal microscope and flow cytometry showed that a large number of nanoparticles weretargeted to SKOV-3 cells

binding to αvβ3 receptor

some of which were uptaken by SKOV-3 cells. With the increasingconcentration of targeted nanoparticles

the ultrasound and CT imaging were enhanced significantly in vitro.Conclusion The10-HCPT liquid fluorocarbon targeting nanoparticles are successfully prepared

and the encapsulation and loading capacityof 10-HCPT are both high. The nanoparticles have strongly specific affinity on the SKOV-3 cells and owned enhancedultrasound/CT imaging in vitro.