Inhibitory effect of focused ultrasound on vulvar skin fibrosis in lichen simplex chronicus rats and its mechanism

LIU Yao; FAN Yijin; QIN Yi; LI Chengzhi

doi:10.16245/j.cnki.issn1008-6978.2020.08.001

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Inhibitory effect of focused ultrasound on vulvar skin fibrosis in lichen simplex chronicus rats and its mechanism

更新时间：2021-10-08

- Inhibitory effect of focused ultrasound on vulvar skin fibrosis in lichen simplex chronicus rats and its mechanism
- Journal of Clinical Ultrasound in Medicine Vol. 22, Issue 8, Pages: 561-565(2020)
- 作者机构：
  
  重庆医科大学生物医学工程学院超声医学工程国家重点实验室重庆市生物医学工程学重点实验室重庆市微无创医学协同创新中心
- 作者简介：
- 基金信息：
- DOI：10.16245/j.cnki.issn1008-6978.2020.08.001
  CLC： R711.7;R445.1
- Published：2020，
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[1]刘瑶,范艺巾,秦溢,李成志.聚焦超声抑制慢性单纯性苔藓大鼠外阴皮肤纤维化及其机制研究[J].临床超声医学杂志,2020,22(08):561-565.

LIU Yao, FAN Yijin, QIN Yi, et al. Inhibitory effect of focused ultrasound on vulvar skin fibrosis in lichen simplex chronicus rats and its mechanism. [J]. Journal of Clinical Ultrasound in Medicine 22(8):561-565(2020)
[1]刘瑶,范艺巾,秦溢,李成志.聚焦超声抑制慢性单纯性苔藓大鼠外阴皮肤纤维化及其机制研究[J].临床超声医学杂志,2020,22(08):561-565. DOI： 10.16245/j.cnki.issn1008-6978.2020.08.001.

LIU Yao, FAN Yijin, QIN Yi, et al. Inhibitory effect of focused ultrasound on vulvar skin fibrosis in lichen simplex chronicus rats and its mechanism. [J]. Journal of Clinical Ultrasound in Medicine 22(8):561-565(2020) DOI： 10.16245/j.cnki.issn1008-6978.2020.08.001.

摘要

目的应用聚焦超声治疗慢性单纯性苔藓大鼠

观察大鼠外阴皮肤组织中转化生长因子β（TGF-β）及B淋巴细胞瘤-2（Bcl-2）蛋白表达的变化

探讨聚焦超声治疗慢性单纯性苔藓的作用机制。方法建立40只雌性SD大鼠外阴慢性单纯性苔藓模型

随机分为实验组和对照组

每组各20只

实验组大鼠采用聚焦超声治疗

对照组采用聚焦超声假辐照治疗;另取10只正常SD大鼠为空白对照组。经聚焦超声治疗4周后

观察并比较实验组与对照组治疗后大鼠外阴皮肤恢复情况;Masson染色鉴定各组大鼠真皮浅层胶原纤维密度;免疫组织化学和Western blotting检测各组外阴皮肤组织中TGF-β和Bcl-2蛋白的表达

比较各组大鼠外阴真皮浅层胶原纤维密度、皮肤组织中TGF-β和Bcl-2蛋白表达情况。结果经聚焦超声治疗后

实验组70%（14/20）的大鼠外阴皮肤恢复正常;对照组仅10%（2/20）的大鼠恢复正常

两组治愈率比较差异有统计学意义（P<0.05）。实验组和空白对照组真皮浅层胶原纤维密度均明显高于对照组

差异均有统计学意义（均P<0.05）;实验组与空白对照组比较差异无统计学意义。实验组和空白对照组外阴组织中TGF-β表达低于对照组

Bcl-2蛋白表达高于对照组

差异均有统计学意义（均P<0.05）;空白对照组与实验组比较差异均无统计学意义。结论聚焦超声可能通过影响外阴皮肤组织中TGF-β和Bcl-2蛋白的表达

有效治疗大鼠外阴慢性单纯性苔藓。

Abstract

ObjectiveTo observe the changes of transforming growth factor-β（TGF-β）and B-cell lymphoma-2（Bcl-2）protein expression in vulva skin tissue of rats treated with focused ultrasound

and to explore the mechanism of action offocused ultrasound in the treatment of lichen simplex chronicus（LSC）.MethodsForty female SD rats with LSC were randomlydivided into experimental group and control group

with 20 rats in each group.The rats in the experimental group were treatedwith focused ultrasound

while the control group were treated with focused ultrasound false irradiation.Another 10 normal SD ratswere selected as the blank control group.After 4 weeks of focused ultrasound treatment

the recovery of vulva skin was observedand compared between the experimental group and the control group.Masson staining was used to identify the density of collagenfibers in the superficial dermis of rats in each group.The expression of TGF-β and Bcl-2 protein in vulva skin tissue weredetected by immunohistochemistry and Western blotting.The density of collagen fiber in superficial dermis and the expression ofTGF-β and Bcl-2 protein in vulva skin tissue were compared.ResultsIn experimental group

70%（14/20）experimental ratsreturned to normal.In control group

only 10%（2/20）returned to normal.The difference of cure rate between the two groups wasstatistically significant（P<0.05）.The density of collagen fibers in the superficial dermis of the experimental group and blank control group were significantly higher than that of control group（both P<0.05）

while there was no significant difference betweenexperimental group and blank control group.The expression of TGF-β protein in vulvar tissues of experimental group and blankcontrol group were significantly lower than that of the control group

while the expression of Bcl-2 protein were higher than thatof the control group（all P<0.05）. There was no significant difference between experimental group and blank control group.ConclusionFocused ultrasound may effectively treat rats with LSC by affecting the expression of TGF-β and Bcl-2 protein invulva skin tissue.

关键词

聚焦超声慢性单纯性苔藓外阴转化生长因子βB淋巴细胞瘤-2大鼠

Keywords

Focused ultrasoundLichen simplex chronicusvulvarTransforming growth factor βB-celllymphoma-2Rat

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