Experimental study of ultrasonic-targeted nanobubbles loading galectin-7-siRNA for inhibiting the acute rejection of rat transplantation hearts

WANG Zhuo; ZHAO Bingbing; LI Shouqiang; YU Dandan; ZHAO Chen; JIANG Shuangquan; LENG Xiaoping

doi:10.16245/j.cnki.issn1008-6978.2020.04.001

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Experimental study of ultrasonic-targeted nanobubbles loading galectin-7-siRNA for inhibiting the acute rejection of rat transplantation hearts

更新时间：2021-10-08

- Experimental study of ultrasonic-targeted nanobubbles loading galectin-7-siRNA for inhibiting the acute rejection of rat transplantation hearts
- Journal of Clinical Ultrasound in Medicine Vol. 22, Issue 4, Pages: 241-245(2020)
- 作者机构：
  
  1. 哈尔滨医科大学附属第二医院超声医学科
  2. 黑龙江中医药大学附属第二医院超声科
- 作者简介：
- 基金信息：
- DOI：10.16245/j.cnki.issn1008-6978.2020.04.001
  CLC： R54;R445.1
- Published：2020，
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[1]王卓,赵冰冰,李守强,于丹丹,赵晨,姜双全,冷晓萍.携galectin-7-siRNA超声纳泡靶向治疗大鼠心脏移植急性排斥反应的实验研究[J].临床超声医学杂志,2020,22(04):241-245.

WANG Zhuo, ZHAO Bingbing, LI Shouqiang, et al. Experimental study of ultrasonic-targeted nanobubbles loading galectin-7-siRNA for inhibiting the acute rejection of rat transplantation hearts. [J]. Journal of Clinical Ultrasound in Medicine 22(4):241-245(2020)
[1]王卓,赵冰冰,李守强,于丹丹,赵晨,姜双全,冷晓萍.携galectin-7-siRNA超声纳泡靶向治疗大鼠心脏移植急性排斥反应的实验研究[J].临床超声医学杂志,2020,22(04):241-245. DOI： 10.16245/j.cnki.issn1008-6978.2020.04.001.

WANG Zhuo, ZHAO Bingbing, LI Shouqiang, et al. Experimental study of ultrasonic-targeted nanobubbles loading galectin-7-siRNA for inhibiting the acute rejection of rat transplantation hearts. [J]. Journal of Clinical Ultrasound in Medicine 22(4):241-245(2020) DOI： 10.16245/j.cnki.issn1008-6978.2020.04.001.

摘要

目的采用超声靶向微泡破坏技术介导galectin-7-siRNA转染

抑制大鼠移植心脏galectin-7表达

以达到靶向治疗心脏移植后急性排斥反应的目的。方法制备携galectin-7-siRNA的ICAM-1靶向纳泡;构建大鼠同系移植（ISO）及异系移植（ALLO）心脏模型

并分为4组:ISO+磷酸盐缓冲液（PBS）组、ALLO+PBS+低功率聚焦超声（LIFU）组、ALLO+微泡（NBs）组及ALLO+NBs+LIFU组。于移植后第1、3、5、7天分别行超声靶向微泡定位释放治疗

治疗后取材比较各组心脏galectin-7表达及急性排斥反应分级。结果制备的纳泡平均粒径（221.25±34.21）nm

平均电位（51.32±2.21）m V;galectin-7-siRNA携带率71.0%。ALLO+NBs+LIFU组galectin-7蛋白表达均低于ALLO+PBS+LIFU组和ALLO+NBs组（均P<0.05）

ALLO+NBs+LIFU组急性排斥反应分级均明显低于ALLO+PBS+LIFU组及ALLO+NBs组（均P<0.05）。结论超声引导下携galectin-7-siRNA靶向微泡爆破治疗能降低大鼠移植心脏galectin-7表达

抑制急性排斥反应发生。

Abstract

Objective To inhibit the expression of galectin-7 and the occurrence of acute rejection after transplantation using galectin-7-siRNA-loaded nanobubbles combined with ultrasound-targeted microbubbles destruction（UTMD） technology.Methods The galectin-7-siRNA-loaded and targeted ICAM-1 nanobubbles were prepared and the rat heart transplantation model was constructed.Isograft（ISO） and Allograft（ALLO） of rat heart models were constructed and divided into 4 groups

including ISO+PBS group

ALLO+PBS+LIFU group

ALLO+NBs group and ALLO+NBs+LIFU group.UTMD were performed at 1

7 d after transplantation.In addition

the galectin-7 expression and acute rejection grading in each group of transplanted hearts were compared.Results The average particle size of NBs was（221.25±34.21）nm and the average potential was（51.32±2.21）mV.The carrying rate of galectin-7-siRNA was 71.0%.The expression of galectin-7 protein in the ALLO+NBs+LIFU group was lower than that in ALLO+PBS+LIFU group and ALLO+NBs group（both P<0.05）

and the level of acute rejection was significantly lower in the ALLO+NBs+LIFU group than that in the ALLO+PBS+LIFU group and ALLO+NBs group（both P<0.05）.Conclusion The galectin-7-siRNA-loaded targeted NBs combined with UTMD can reduce the expression of galectin-7 in the transplanted heart of rats and inhibit the occurrence of acute graft rejection.

关键词

超声靶向纳泡心脏移植基因治疗

Keywords

UltrasoundTargeted nanobubbleHeart transplantionGene therapy

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