Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice

Qixiang Song; Chen Ye; Haisong Tan; Zhensheng Zhang; Chuanliang Xu; Yinghao Sun

doi:10.16016/j.1000-5404.201608088

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Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice

Basic Medicin | 更新时间：2020-08-05

- Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice
- J Third Mil Med Univ Vol. 39, Issue 2, Pages: 179-184(2017)
- 作者机构：
  
  200433 上海, 第二军医大学长海医院泌尿外科
- 作者简介：
- 基金信息：
  
  Supported by the National Natural Science Foundation for Young Scholars of China(81500579)
- DOI：10.16016/j.1000-5404.201608088
  CLC： R-334;R334;R337.5
- Published：30 January 2017，
  
  Received：18 August 2016，
  
  Accepted：17 September 2016
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Qixiang Song, Chen Ye, Haisong Tan, et al. Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice. [J]. J Third Mil Med Univ 39(2):179-184(2017)
DOI：

Qixiang Song, Chen Ye, Haisong Tan, et al. Development and validation of simultaneous recording of cystometrography and external urethral sphincter electromyography in mice. [J]. J Third Mil Med Univ 39(2):179-184(2017) DOI： 10.16016/j.1000-5404.201608088.

摘要

目的

建立小鼠膀胱内压力(cystometrography

CMG)与尿道外括约肌肌电图(external urethral sphincter electromyography

EUS EMG)同步测定的方法，并验证其可靠性。

方法

将12只年龄、体质量相仿的雌性小鼠麻醉后予以耻骨上膀胱造漏并植入导管，然后进行CMG和漏尿点压(leak point pressure

LPP)测定与同步EUS EMG记录。完毕后离断双侧阴部神经，再次重复CMG、LPP及EUS EMG测定，以检测EUS EMG信号的准确性。神经损伤前后计量资料之间的比较采用配对

检验。

结果

在未损伤阴部神经时，EUS EMG在LPP处的振幅和频率较基础膀胱压时的振幅和频率有明显的增加(

＜0.001)。定量分析排尿期所出现的“EUS EMG间断性扑动”信号发现：振幅与频率分别为 (70.4±15.2)μV和(393.8±118.9)Hz，单次扑动的持续时间：(85.60±6.21)ms，两个扑动信号之间的间隔时间：(222.0±44.2) ms。而双侧阴部神经离断后，EUS EMG信号几乎完全不能被检测到，说明肌电图信号的真实性。另外，LPP、顶点膀胱压与排尿时的最大膀胱压在离断双侧阴部神经后出现了显著的降低，分别为。

结论

成功建立并验证了CMG与EUS EMG同步测定在小鼠模型上的可行性。

Abstract

Objective

To establish and validate simultaneous recording of cystometrography (CMG) and external urethral sphincter electromyography (EUS EMG) in mice.

Methods

Twelve female

age-matched C57BL/6 mice were anesthetized

and the pudendal canal was located bilaterally

followed by implantation of a polyethylene catheter into the bladder for simultaneous recording of CMG

leak point pressure (LPP) and EUS EMG. After bilateral pudendal nerve transection (PNT)

the LPP and EUS EMG recording was repeated on the same mice. Statistical comparisons of quantitative data before and after PNT were made using a paired

-test with

＜0.05.

Results

When the pudendal nerve was intact

EUS EMG amplitude and frequency were increased significantly in response to LPP testing (from 38.9±8.4 μV to 111.7±19.4 μV and from 37.6±9.5 Hz to 142.6±15.0 Hz

respectively

＜0.001). Quantitative analysis of EUS EMG bursting activity during voiding was characterized with intrabursting amplitude of 70.4±15.2 μV

intrabursting firing rate of 393.8±118.9 Hz

interbursting interval of 222.0± 44.2 ms and bursting duration of 85.60±6.21 ms. Bilateral PNT eliminated EMG activity

which verified the recording quality. Besides

LPP

peak bladder pressure and maximum bladder pressure during voiding were also significantly reduced after PNT (16.4±1.2

7.4±0.7 cmH

＜0.001; 26.7±1.7

15.9±1.1 cmH

＜0.001; 35.5±2.0

22.8±1.1 cmH

＜0.001

respectively).

Conclusion

Simultaneous recording of CMG and EUS EMG in mice is feasible

so it can be used as functional outcome in the investigation of lower urinary tract symptoms.

关键词

下尿路症状阴部神经动物模型尿动力学

Keywords

lower urinary tract symptomspudendal nerveanimal modelurodynamics

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