Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells

Liqin Xia; Zhongming Feng; Xu Chen; Yan Li; Yanmei Wang; Dingxi Lu; Jiaoyang Gu; Jianguo Sun

doi:10.16016/j.1000-5404.201608007

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Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells

Basic Medicin | 更新时间：2020-08-05

- Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells
- J Third Mil Med Univ Vol. 39, Issue 2, Pages: 130-136(2017)
- 作者机构：
  
  400037 重庆, 第三军医大学新桥医院肿瘤科
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81272910)
- DOI：10.16016/j.1000-5404.201608007
  CLC： R394-33;R394.3;R737.9
- Published：30 January 2017，
  
  Received：1 August 2016，
  
  Accepted：30 September 2016
扫描看全文
Liqin Xia, Zhongming Feng, Xu Chen, et al. Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells. [J]. J Third Mil Med Univ 39(2):130-136(2017)
DOI：

Liqin Xia, Zhongming Feng, Xu Chen, et al. Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells. [J]. J Third Mil Med Univ 39(2):130-136(2017) DOI： 10.16016/j.1000-5404.201608007.

摘要

目的

网络筛选乳腺癌关键微小RNAs(microRNAs，miRNAs)，并探讨miR-106a-5p对乳腺癌细胞侵袭及迁移能力的影响。

方法

通过TargetScan和miRanda等工具，构建miRNAs与靶基因以及显著靶向性功能(gene ontology，GO)的调控网络，筛选对乳腺癌有关键调控作用的miRNAs；利用miR-106a-5p agomir及miR-106a-5p antagomir转染MCF-7、T47D乳腺癌细胞株，建立乳腺癌细胞中miR-106a-5p的过表达及敲低体系，Transwell侵袭实验、Transwell迁移实验、划痕实验检测miR-106a-5p对乳腺癌细胞的侵袭、迁移能力的影响。

结果

在miRNAs-gene-network和miRNAs-GO-network两个网络中调控维度最高的miRNAs基本一致，其中miR-106a-5p是调控维度最高的miRNAs之一；过表达miR-106a-5p显著抑制乳腺癌细胞的侵袭能力和迁移能力(

＜0.05)，敲低miR-106a-5p则显著增加乳腺癌细胞的侵袭能力和迁移能力(

＜0.05)。

结论

miR-106a-5p是乳腺癌miRNAs调控网络中的关键miRNAs之一，具有抑制乳腺癌细胞的侵袭及迁移能力的生物学功能。

Abstract

Objective

To screen key miRNAs related with breast cancer and explore the modulating effect of miR-106a-5p on the invasion and migration of breast cancer cells.

Methods

TargetScan and miRanda softwares were employed to predict the targets of miRNA array in breast cancer cells. The miRNAs-gene-network and miRNAs-GO-network were established based on gene ontology (GO) analysis. The overexpression and knockdown of miR-106a-5p were established by transfection of miR-106a-5p agomir and miR-106a-5p antagomir into MCF-7 and T47D breast cancer cells

respectively. The invasion and migration of breast cancer cells were tested by Transwell invasion assay

Transwell migration assay and scratch assay.

Results

MiR-106a-5p was one of miRNAs with the highest regulating degree in both miRNAs-gene-network and miRNAs-GO-network. The expression of miR-106a-5p in breast cancer cells was significantly lower than that in normal mammary cells (

＜0.05). The overexpression of miR-106a-5p inhibited the invasion and migration of MCF-7 and T47D breast cancer cells (

＜0.05). On the contrary

the knockdown of miR-106a-5p promoted the invasion and migration of breast cancer cells (

＜0.05).

Conclusion

miR-106a-5p is a key miRNA in the regulation network of breast cancer miRNAs

and can inhibit the invasion and migration of breast cancer cells.

关键词

miR-106a-5p生物信息学乳腺癌细胞侵袭迁移

Keywords

miR-106a-5pbioinformatics analysisbreast cancer cellsinvasionmigration

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