Efficacy of dihydromyricetin in treatment of dextran sulfate induced colitis in mice

Rong Wei; Peirong Li; Zhiping Wu; Guoqing Qi; Ruixia Xie; Weihong Hang; Jun Yan; Dekui Zhang

doi:10.16016/j.1000-5404.201607166

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Efficacy of dihydromyricetin in treatment of dextran sulfate induced colitis in mice

Basic Medicin | 更新时间：2020-08-05

- Efficacy of dihydromyricetin in treatment of dextran sulfate induced colitis in mice
- J Third Mil Med Univ Vol. 39, Issue 2, Pages: 169-174(2017)
- 作者机构：
  
  1.730030 兰州, 兰州大学第二医院消化科
  2.730030 兰州, 甘肃省人民医院西院消化科
  3.730030 兰州, 甘肃省消化系肿瘤重点实验室
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81272661);基金项目(1506RJZA204)
- DOI：10.16016/j.1000-5404.201607166
  CLC： R284.1;R285.6;R574.62
- Published：30 January 2017，
  
  Received：24 July 2016，
  
  Accepted：14 October 2016
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Rong Wei, Peirong Li, Zhiping Wu, et al. Efficacy of dihydromyricetin in treatment of dextran sulfate induced colitis in mice. [J]. J Third Mil Med Univ 39(2):169-174(2017)
DOI：

Rong Wei, Peirong Li, Zhiping Wu, et al. Efficacy of dihydromyricetin in treatment of dextran sulfate induced colitis in mice. [J]. J Third Mil Med Univ 39(2):169-174(2017) DOI： 10.16016/j.1000-5404.201607166.

摘要

目的

观察二氢杨梅素(dihydromyricetin， DHM)治疗溃疡性结肠炎(ulcerative colitis， UC)的效果并探讨其机制。

方法

60只BALB/c小鼠采用随机数字表法分为正常组，模型组，DHM低剂量、中剂量、高剂量组，5-氨基水杨酸组。分组当日给予正常组小鼠蒸馏水自行饮用，其余组小鼠给予4%葡聚糖硫酸钠(DSS)水溶液7 d诱导急性UC模型。于饮用DSS当日开始，用DHM各剂量组及5-氨基水杨酸组按各组拟定剂量分别进行灌胃处理，连续给药14 d。对比各组疾病活动指数(DAI)、结肠长度、脾脏指数的变化、结肠组织学评分，肠组织TLR2、TLR4和NF-κB 的活性以及炎性细胞因子IL-1β、TNF-α、IL-6表达及MPO、NO、SOD的活性变化。

结果

与模型组比较，DHM可显著降低结肠组织中TLR2、TLR4、NF-κB的表达，减少炎性因子如IL-1β、TNF-α、IL-6水平，同时还能够减少肠组织中MPO、NO活性及增加SOD的活性。其中DHM高剂量组优于低、中剂量组，差异有统计学意义(

＜0.05)，治疗效果最明显，其与5-氨基水杨酸组相比差异无统计学意义(

＞0.05)，治疗效果相当。

结论

DHM可显著缓解DSS诱导的小鼠结肠炎症，其作用机制可能与抗炎、抗氧化有关。

Abstract

Objective

To observe the curative effect of dihydromyricetin(DHM) in treating ulcerative colitis (UC) and to explore its mechanism.

Methods

Sixty BALB/c mice were randomly divided into normal group

model group

low-

middle-

and high-dosed DHM treatment groups and 5- ASA control group

with 10 mice in each group. The normal group was given distilled water to drink

and the other groups were given 4% dextran sodium sulfate (DSS) solution for 7 d to induce the acute UC model. At the beginning of the day of drinking DSS

the drugs were given to the mice of DHM and 5-ASA treatment groups respectively through intragastric continuous administration for 14 d. Disease activity index (DAI)

colon length

spleen index changes

histological scores

activities of TLR2

TLR4 and NF-kappa B

expression levels of inflammatory cytokines IL-1β

TNF-α

IL-6 and activities of MPO

SOD in intestinal tissue were compared among the groups.

Results

Compared with model group

DHM could significantly reduce the expression of TLR2

TLR4

NF-κB in colon tissue

reduce the levels of inflammatory cytokines such as IL-1β

TNF-α and IL-6

and also reduce the production of MPO and NO and increase SOD activity in intestinal tissue. The high-dosed treatment group had better results than low- and middle-dosed treatment groups

with significant with differences (

＜0.05)

and the treatment effect was most obvious and was equivalent to the action of 5-ASA (

0.05).

Conclusion

DHM can significantly alleviate the DSS induced colitis in mice

and its mechanism may be related to its anti-inflammatory and antioxidant activities.

关键词

二氢杨梅素葡聚糖硫酸钠结肠炎TLR2TLR4

Keywords

dihydromyricetindextran sulphate sodium saltulcerative colitisToll-like receptor 2Toll-like receptor 4

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