基因芯片方法研究苦参碱抗白血病细胞增殖机制

张永清; 黄高升; 王哲; 冯骥良; 郭英

doi:10.16016/j.1000-5404.2003.14.019

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基因芯片方法研究苦参碱抗白血病细胞增殖机制

更新时间：2026-01-09

- 基因芯片方法研究苦参碱抗白血病细胞增殖机制
- Journal of Army Medical University Vol. 25, Issue 14, (2003)
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- DOI：10.16016/j.1000-5404.2003.14.019
  CLC： R733.7
- Online First：30 July 2003，
  
  Published：2003
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张永清,黄高升,王哲,冯骥良,郭英,基因芯片方法研究苦参碱抗白血病细胞增殖机制[J].陆军军医大学学报(原第三军医大学学报),2003,25(14):0-.

张永清, 黄高升, 王哲, et al. 基因芯片方法研究苦参碱抗白血病细胞增殖机制[J]. Journal of Army Medical University, 2003, 25(14).
张永清,黄高升,王哲,冯骥良,郭英,基因芯片方法研究苦参碱抗白血病细胞增殖机制[J].陆军军医大学学报(原第三军医大学学报),2003,25(14):0-. DOI：10.16016/j.1000-5404.2003.14.019

张永清, 黄高升, 王哲, et al. 基因芯片方法研究苦参碱抗白血病细胞增殖机制[J]. Journal of Army Medical University, 2003, 25(14). DOI： 10.16016/j.1000-5404.2003.14.019.

摘要

目的　探索苦参碱抗白血病细胞增殖的机制。方法　提取苦参碱诱导前后白血病细胞株K5 62总RNA

经逆转录合成cDNA探针后与人的细胞周期相关基因芯片杂交

用ESTblot软件分析杂交结果。结果　经苦参碱诱导后K5 62细胞 111个周期相关基因中

有CCNB1

cyclinD1

PCNA

TFDP1等 15个基因表达显著下调

CDKN2D

E2F5等 5个基因表达上调

Westernblotting方法证实了PCNA基因表达改变。结论　苦参碱的抗白血病细胞增殖作用与PCNA

P19 INK4D等多个细胞周期相关基因表达变化有关。

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