核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用

姚伟; 陆俊羽; 钱桂生; 杨晓静

doi:10.16016/j.1000-5404.2003.05.006

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核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用

更新时间：2026-01-09

- 核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用
- Journal of Army Medical University Vol. 25, Issue 5, (2003)
- 作者机构：
- 作者简介：
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- DOI：10.16016/j.1000-5404.2003.05.006
  CLC： R560.2
- Online First：15 March 2003，
  
  Published：2003
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姚伟,陆俊羽,钱桂生,杨晓静,核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用[J].陆军军医大学学报(原第三军医大学学报),2003,25(05):0-.

姚伟, 陆俊羽, 钱桂生, et al. 核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用[J]. Journal of Army Medical University, 2003, 25(5).
姚伟,陆俊羽,钱桂生,杨晓静,核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用[J].陆军军医大学学报(原第三军医大学学报),2003,25(05):0-. DOI：10.16016/j.1000-5404.2003.05.006

姚伟, 陆俊羽, 钱桂生, et al. 核酶对大鼠肺动脉平滑肌NHE-1活性及细胞增殖的作用[J]. Journal of Army Medical University, 2003, 25(5). DOI： 10.16016/j.1000-5404.2003.05.006.

摘要

目的　探讨锤头状核酶对大鼠肺动脉平滑肌细胞Na+ H+ 交换器 1表达和活性、pHi的影响

及其与细胞增殖的关系。方法　构建含锤头状核酶序列的pLXSN反转录病毒重组载体

将其转染体外培养的大鼠肺动脉平滑肌细胞

G418筛选后获取含有重组载体的细胞克隆。检测细胞NHE 1mRNA表达、pHi、pHi恢复速率、2 2 Na摄取量和3H TdR掺入量变化。结果　与转染pLXSN空载体的细胞和正常对照组细胞比较

转染了重组载体的肺动脉平滑肌细胞中NHE 1mRNA表达、2 2 Na摄取量减少

同时伴有pHi降低、pHi恢复速率下降和3H TdR掺入量减少。结论　所设计的锤头状核酶可对NHE 1mRNA进行特异性切割

减少其表达

使其活性下降

从而诱导细胞酸化

抑制肺动脉平滑肌细胞增殖

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Department of Pulmonary and Critical Care Medicine, Institute of Respiratory Diseases, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Provmce

Department of Orthopedics, the Second Affiliated Hospital of Chongqing Medical University

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Department of Otolaryngology, the First Affiliated Hospital of Chongqing Medical University

Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Southwest Medical University

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