NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响

陆俊羽; 姚伟; 钱桂生; 吴国明

doi:10.16016/j.1000-5404.2001.11.014

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NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响

更新时间：2026-01-09

- NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响
- Journal of Army Medical University Vol. 23, Issue 11, (2001)
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- DOI：10.16016/j.1000-5404.2001.11.014
  CLC： R329.2
- Online First：30 November 2001，
  
  Published：2001
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陆俊羽,姚伟,钱桂生,吴国明,NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2001,23(11):0-.

陆俊羽, 姚伟, 钱桂生, et al. NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响[J]. Journal of Army Medical University, 2001, 23(11).
陆俊羽,姚伟,钱桂生,吴国明,NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2001,23(11):0-. DOI：10.16016/j.1000-5404.2001.11.014

陆俊羽, 姚伟, 钱桂生, et al. NHE-1核酶基因转染对大鼠肺动脉平滑肌细胞凋亡的影响[J]. Journal of Army Medical University, 2001, 23(11). DOI： 10.16016/j.1000-5404.2001.11.014.

摘要

目的　探讨NHE 1核酶基因转染对大鼠肺动脉平滑肌细胞 (PASMC)凋亡的影响。方法　构建NHE 1核酶基因逆转录病毒载体

转染体外培养的大鼠PASMC

应用半定量RT PCR法 (sqRT PCR)测定细胞内NHE 1mRNA的表达、BCECF法测定pHi、流式细胞仪测细胞周期、电镜及原位细胞凋亡检测 (TUNEL法 )观察细胞凋亡情况。结果　转染NHE 1核酶基因的细胞

NHE 1mRNA表达量及pHi较空载体转染细胞及未转染细胞显著降低

细胞凋亡率显著增加

电镜示细胞凋亡

凋亡小体形成。结论　NHE 1核酶基因转染可通过NHE 1抑制、细胞内酸化

显著诱导肺动脉平滑肌细胞凋亡。

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