Regulation of Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cells by Solasonine Through MAPK/ERK Signaling Pathway

LIU Yingqi; SHI Zhe; SUN Shugang; ZHOU Liyuan; YANG Yong; WANG Xiaohui

doi:10.14009/j.issn.1672-2124.2023.02.011

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Regulation of Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cells by Solasonine Through MAPK/ERK Signaling Pathway

更新时间：2023-04-14

- Regulation of Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cells by Solasonine Through MAPK/ERK Signaling Pathway
- Issue 2, Pages: 182-186(2023)
- 作者机构：
  
  1. 河北工程大学附属医院普外科
  2. 河北工程大学附属医院妇科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2023.02.011
  CLC： R285
- Published：2023，
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LIU Yingqi, SHI Zhe, SUN Shugang, et al. Regulation of Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cells by Solasonine Through MAPK/ERK Signaling Pathway. [J]. (2):182-186(2023)
DOI：

LIU Yingqi, SHI Zhe, SUN Shugang, et al. Regulation of Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cells by Solasonine Through MAPK/ERK Signaling Pathway. [J]. (2):182-186(2023) DOI： 10.14009/j.issn.1672-2124.2023.02.011.

摘要

目的：探讨澳洲茄碱对肝癌细胞增殖活性的影响及对丝裂原激活的蛋白激酶（MAPK）/胞外信号调节激酶（ERK）信号通路的调节作用。方法：取对数生长期的HepG2细胞随机分为对照组、澳洲茄碱组（以50μmol/L澳洲茄碱处理细胞）、激活剂组[以20μmol/L异丙肾上腺素（ISO）处理细胞]和激活剂+澳洲茄碱组（20μmol/L ISO处理细胞24 h后，以50μmol/L澳洲茄碱处理）

24 h后采用四甲基偶氮唑盐法检测细胞存活率，采用划痕实验检测细胞迁移能力，采用Transwell试验检测细胞侵袭能力，采用蛋白质印迹法检测磷酸化ERK（p-ERK）、ERK、丝裂原激活的蛋白激酶激酶（MEK）和磷酸化MEK（p-MEK）蛋白相对表达量。结果：与0μmol/L澳洲茄碱比较，10、20、30、40及50μmol/L澳洲茄碱均可降低细胞存活率，差异均有统计学意义（P＜0.05）

且具浓度依赖性。与对照组比较，澳洲茄碱组细胞存活率、划痕愈合率，p-ERK、p-MEK蛋白相对表达量降低，侵袭细胞数减少；激活剂组细胞划痕愈合率，p-ERK、p-MEK蛋白相对表达量升高，侵袭细胞数增多，差异均有统计学意义（P＜0.05）。与澳洲茄碱组比较，激活剂+澳洲茄碱组细胞存活率、划痕愈合率，p-ERK、p-MEK蛋白相对表达量升高，侵袭细胞数增多，差异均有统计学意义（P＜0.05）。与激活剂组比较，激活剂+澳洲茄碱组细胞存活率、划痕愈合率，p-ERK、p-MEK蛋白相对表达量降低，侵袭细胞数减少，差异均有统计学意义（P＜0.05）。结论：澳洲茄碱可降低肝癌细胞增殖、迁移和侵袭能力，其可能是通过抑制MAPK/ERK信号通路发挥调控作用。

Abstract

OBJECTIVE: To investigate the effects of solasonine on the proliferative activity of hepatoma cells and the regulation of mitogen-activated protein kinase/extracellular signaling regulation kinase（MAPK/ERK） signaling pathway. METHODS: HepG2 cells in logarithmic growth phase were randomly divided into the control group

solasonine group（cells treated with 50 μmol/L solasonine）

activator group（cells treated with 20 μmol/L ISO） and activator + solasonine group（cells treated with 50 μmol/L solasonine after 24 h of treatment with 20 μmol/L ISO）. After 24 h

MTT assay was used to detect cell viability

scratch assay was used to detect cell migration

transwell assay was used to detect cell invasion

and Western blotting was used to detect the relative expression levels of p-ERK

ERK

mitogen activated protein kinase kinase（MEK） and p-MEK protein. RESULTS: Compared with the 0 μmol/L solasonine group

the cell viability of 10

40 and 50 μmol/L solasonine group decreased significantly（P<0.05）

the difference was concentration-dependent. Compared with the control group

the cell survival rate

scratch healing rate and relative expression levels of p-ERK and p-MEk protein and number of invasive cells in the solasonine group decreased

while the scratch healing rate and relative expression levels of p-ERK and p-MEk protein and number of invasive cells in the activator group increased

with statistically significant differences（P<0.05）. Compared with the solasonine group

the cell survival rate

scratch healing rate and relative expression levels of p-ERK and p-MEk protein and number of invasive cells increased in activator + solasonine group

with statistically significant difference（P<0.05）. Compared with the activator group

the cell survival rate

scratch healing rate and relative expression levels of p-ERK and p-MEk protein and number of invasive cells decreased in the activator + solasonine group

with statistically significant difference（P<0.05）. CONCLUSIONS: Solasonine can reduce the proliferation

migration and invasion of hepatocellular carcinoma cells

possibly by inhibiting MAPK/ERK signaling pathway.

关键词

肝癌澳洲茄碱增殖迁移侵袭丝裂原激活的蛋白激酶/胞外信号调节激酶

Keywords

Hepatocellular carcinomaSolanineProliferationMigrationInvasionMitogen-activated protein kinase/extracellular signal-regulated kinase

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