Tatarinan O Inhibits TGF-&#946;1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway

WU Di; CHEN Yinghua; YANG Yan

doi:10.14009/j.issn.1672-2124.2023.01.014

您当前的位置：

首页 >

文章列表页 >

Tatarinan O Inhibits TGF-β1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway

更新时间：2023-04-14

- Tatarinan O Inhibits TGF-β1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway
- Issue 1, Pages: 65-69(2023)
- 作者机构：
  
  1. 重庆市急救医疗中心药剂科
  2. 重庆市急救医疗中心肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2023.01.014
  CLC： R734.2
- Published：2023，
扫描看全文
WU Di, CHEN Yinghua, YANG Yan. Tatarinan O Inhibits TGF-β1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway. [J]. (1):65-69(2023)
DOI：

WU Di, CHEN Yinghua, YANG Yan. Tatarinan O Inhibits TGF-β1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway. [J]. (1):65-69(2023) DOI： 10.14009/j.issn.1672-2124.2023.01.014.

摘要

目的：探讨石菖蒲中木脂素单体化合物Tatarinan O（TO）对转化生长因子β1（TGF-β1）诱导的非小细胞肺癌A459细胞上皮间质转化（EMT）及其迁移的影响。方法：通过TGF-β1诱导A549细胞EMT的发生。采用蛋白质印迹法、免疫荧光法检测E-钙黏蛋白（E-cadherin）、波形蛋白（Vimentin）、SMAD2蛋白和磷酸化SMAD2（p-SMAD2）蛋白的表达，以及SMAD2的核转位行为；采用划痕实验检测细胞迁移能力。结果：TO能够在A549细胞上抑制TGF-β1诱导的SMAD2的磷酸化，且呈剂量依赖性（P＜0.05）

并阻止其核转位。在25、50μmol/L的TO作用下，Vimentin表达明显降低，E-cadherin表达明显升高，与0μmol/L的TO相比，差异有统计学意义（P＜0.05）

并进一步抑制A549细胞迁移行为。结论：TO可通过SMAD2信号通路抑制TGF-β1诱导的非小细胞肺癌A549细胞的EMT

降低其迁移能力。

Abstract

OBJECTIVE: To investigate the effect of Tatarinan O（TO）

a lignans monomeric compound in Acorus calamus

on transforming growth factor-β1（TGF-β1）-induced epithelial mesenchymal transition（EMT） and its migration of non-small cell lung cancer A459 cells. METHODS: The onset of EMT in A549 cells was induced by TGF-β1. The expression of E-cadherin

Vimentin

SMAD2 protein and phosphorylated SMAD2（p-SMAD2） protein

and the nuclear translocation behavior of SMAD2 were detected by using Western blotting and immunofluorescence assay; the cell migration ability was detected by scratch assay. RESULTS: TO was able to inhibit TGF-β1-induced phosphorylation of SMAD2 on A549 cells

which presented a dose-dependent（P<0.05） and prevented its nuclear translocation. The Vimentin expression was significantly reduced and the E-cadherin expression was significantly increased under the action of TO at 25 and 50 μmol/L

with statistically significant differences（P<0.05） compared with TO at 0 μmol/L

which further inhibited the migratory behavior of A549 cells. CONCLUSIONS: TO can inhibit TGF-β1-induced EMT in non-small cell lung cancer A549 cells through SMAD2 signaling pathway

and reduce its migratory ability.

关键词

石菖蒲木脂素类单体上皮间质转化肿瘤细胞迁移

Keywords

Acorus calamusLignans monomeric compoundEpithelial-mesenchymal transitionCancer cell migration

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Danshensu on Src/FAK Signaling Pathway and Epithelial-Mesenchymal Transition in Bladder Cancer 5637 Cells

Related Author

LUO Renxiu

GAO Hong

CUI Dawei

LYU Xiaolei

Related Institution

Dept.of Urology Surgery, Tangshan Fengnan District Hospital

Dept.of Urology Surgery, Tangshan Workers’ Hospital

⁰

Tatarinan O Inhibits TGF-β1 Induced Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer A549 Cells via SMAD Signaling Pathway

DOI：10.14009/j.issn.1672-2124.2023.01.014

摘要

Abstract

关键词

Keywords

references