Mechanism of Mulberry Leaf-Mulberry Parasite in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Bioinformatics

LI Dan; JIANG Yuanyuan; HUANG Chenxi; LIU Chengsheng; FAN Wei; XUE Song; WU Guoqing

doi:10.14009/j.issn.1672-2124.2023.01.001

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Mechanism of Mulberry Leaf-Mulberry Parasite in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Bioinformatics

更新时间：2023-04-14

- Mechanism of Mulberry Leaf-Mulberry Parasite in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Bioinformatics
- Issue 1, Pages: 1-8(2023)
- 作者机构：
  
  1. 江西中医药大学研究生院
  2. 江西中医药大学附属医院肾病科/江西省中医药重点研究室-慢性肾衰病重点研究室
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2023.01.001
  CLC： R285
- Published：2023，
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LI Dan, JIANG Yuanyuan, HUANG Chenxi, et al. Mechanism of Mulberry Leaf-Mulberry Parasite in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Bioinformatics. [J]. (1):1-8(2023)
DOI：

LI Dan, JIANG Yuanyuan, HUANG Chenxi, et al. Mechanism of Mulberry Leaf-Mulberry Parasite in the Treatment of Diabetic Nephropathy Based on Network Pharmacology and Bioinformatics. [J]. (1):1-8(2023) DOI： 10.14009/j.issn.1672-2124.2023.01.001.

摘要

目的：运用网络药理学和生物信息学探讨桑叶-桑寄生治疗糖尿病肾病（DN）的潜在机制。方法：从中药系统药理学数据库与分析平台、GeneCards等数据库获取桑叶-桑寄生的成分及DN的基因，从GEO数据库筛选DN与健康人差异基因，利用Cytoscape、R语言等将共同基因、差异基因进行基因本体（GO）功能富集分析、京都基因与基因组百科全书（KEGG）通路富集分析，将两者KEGG的结果取交集，利用分子对接方法加以验证。结果：桑叶-桑寄生的有效成分有31种，对应204个基因，DN相关基因3 566个，共同基因139个。GSE142153数据集符合条件，获得113个差异基因。KEGG通路富集分析结果显示，主要涉及的通路为糖尿病并发症中的晚期糖基化终末产物-糖基化终末产物受体信号通路、肿瘤坏死因子信号通路等。分子对接结果显示，山柰酚、槲皮素与环氧合酶2、蛋白激酶B和半胱氨酸-天冬氨酸蛋白酶等具有良好的结合活性。结论：桑叶-桑寄生可通过多靶点、多通路的复杂机制发挥抗炎、维持足细胞稳态等作用来治疗糖尿病肾病。

Abstract

OBJECTIVE: To explore the potential mechanism of mulberry leaf-mulberry parasite in the treatment of diabetic nephropathy（DN） based on network pharmacology and bioinformatics. METHODS: Components of mulberry leaf-mulberry parasite and genes of DN were obtained from Traditional Chinese medicine systems pharmacology database and analysis platform

GeneCards and other databases. The differential genes between DN and the healthy people were screened from the GEO database. Cytoscape and R language were used to perform gene ontology（GO） functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis of common genes and differential genes. Results of KEGG pf two genes were intersected and verified by molecular docking method. RESULTS: There were 31 active components for mulberry leaf-mulberry parasite

which corresponded to 204 genes

3 566 DN-related genes and 139 common genes. The GSE142153 dataset met the criteria and 113 differential genes were obtained. KEGG pathway enrichment analysis showed that the main involved pathways were advanced glycation end products-receptor for advanced glycation end products signaling pathway in diabetic complications

tumor necrosis factor signaling pathway. Results of molecular docking showed that kaferol and quercetin had good binding activity with cyclooxygenase 2

protein kinase B and cysteine aspartic acid protease. CONCLUSIONS: Mulberry leaf-mulberry parasite can treat DN by giving full play to the role of anti-inflammation and the maintaining of podocyte homeostasis through the multi-target and multi-pathway complex mechanisms.

关键词

桑叶-桑寄生糖尿病肾病网络药理学生物信息学

Keywords

Mulberry leaf-mulberry parasiteDiabetic nephropathyNetwork pharmacologyBioinformatics

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