OBJECTIVE: To probe into the metabolic mechanism of Danbei Yifei decoction on idiopathic pulmonary fibrosis rats based on lipidomic technology. METHODS: The rat model of idiopathic pulmonary fibrosis was established by intratracheal injection of bleomycin. Serum lipid changes were detected by ultra-high performance liquid chromatography-performance liquid chromatography-quadrupole electrostatic field orbital well mass spectrometry

and differential ions were identified to search for potential biomarkers. RESULTS: Lipidomics and pathway enrichment analysis showed that the abnormal metabolism of the rats with idiopathic pulmonary fibrosis mainly involved linoleic acid metabolism

α-linolenic acid metabolism

arachidonic acid metabolism

triglyceride metabolism and glycerophospholipid metabolism. ROC curves showed that 11 lipid components were clearly correlated with idiopathic pulmonary fibrosis

with the tendency of different degrees of regression after the intervention of Danbei Yilong decoction. CONCLUSIONS: Danbei Yiku decoction may achieve the treatment of idiopathic pulmonary fibrosis by regulating biomarkers and related pathways

providing a reliable basis for lipid metabolism in idiopathic pulmonary fibrosis.