OBJECTIVE: To establish the method for effective quality evaluation of coptidis rhizoma from different origins based on high performance liquid chromatography fingerprint and chemometrics. METHODS: Merck Purospher Star LP RP-18 endcapped column（250 mm×4.6 mm

5 μm） was adopted. Acetonitrile（A）-0.05 mol/L potassium dihydrogen phosphate（pH was adjusted to 3.0 by phosphoric acid）（B） was used as mobile phase for gradient elution. The flow rate was 1 mL/min

the column temperature was 25 ℃

the sample size was 10 μL

and the detection wavelength was 345 nm. “Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System”（2012 edition） was used to evaluate the similarity

and the common peaks were confirmed and identified. Quality of coptidis rhizoma from different origins was analyzed and evaluated by hierarchical clustering analysis and principal component analysis. RESULTS: Eleven common peaks of coptidis rhizoma from different origins were identified

and 4 common peaks were identified as epistropine（peak No.6）

coptisine（peak No.8）

palmatine（peak No.9） and berberine（peak No.10）

respectively. Similarities of 20 batches of coptidis rhizoma from 4 origins ranged from 0.933 to 0.981. CONCLUSIONS: This analysis method provides basis for the selection of fixed origin of coptidis rhizoma.